中医药导报2023，Vol.29Issue(11)：1-5,5.DOI:10.13862/j.cn43-1446/r.2023.11.001
木犀草苷抑制SNHG14表达改善SW1353骨关节炎细胞模型的实验研究
Inhibition of Luteolin on SNHG14 Expression to Improve SW1353 Osteoarthritis Cell Model
摘要
Abstract
Objective:To observe the protective effect of luteolin(Cynaroside,Cy)on osteoarthritis cell models and to explore its possible mechanism.Methods:SW1353 cells were treated with 0.1％DMSO as well as different concentrations of Cy(0,10,20,30,40,50 μmol/mL).And CCK-8 was used to detect their cytotoxicity and determine the stimulus concentration.SW1353 cells were divided into normal control group,IL-1β group,Cy low-dose group and Cy high-dose group.SW1353 cells were not treated at all in normal control group.SW1353 cells were treated with 10 ng/mL IL-1β in IL-1β group,10 ng/mL IL-1β+10 μmol/mL Cy in Cy low-dose group,and 10 ng/mL IL-1β+20 μmol/mL Cy in luteolin high-dose group for 24 h.RT-qPCR was used to detect the expression levels of metalloproteinase-13(MMP-13)mRNA,interleukin 6(IL-6)mRNA,type Ⅱ collagen(COL2a1)mRNA,proteoglycan(ACAN)mRNA and long non-coding RNA(lnc RNA)SNHG14 mRNA.Western blotting was used to detect the expression of MMP-13 and extracellular matrix COL2a1 and ACAN proteins.Results:The results of CCK-8 showed that Cy(10,20 μmol/mL)showed no significant inhibitory effect on SW1353 cell activity.The IL-1β group showed higher relative expression of MMP-13 mRNA and IL-6 mRNA than normal control group,while lower relative expression of ACAN mRNA and COL2a1 mRNA than normal control group,with statistically significant differences(P＜0.05).The low and high Cy groups showed lower relative expression of MMP-13 mRNA and IL-6 mRNA than IL-1β group,while higher relative expression of ACAN mRNA and COL2a1 mRNA than IL-1β group,with statistically significant differences(P＜0.05).The IL-1β group showed higher relative expression of lncRNA SNHG14 mRNA than normal control group,and the difference was statistically significant(P＜0.01).The low and high Cy groups showed lower relative expression of lncRNA SNHG14 mRNA than IL-1β group,and the difference was statistically significant(P＜0.01).The IL-1β group showed higher relative expression of MMP-13 protein than normal control group,while lower relative expression of ACAN and COL2a1 protein than normal control group,with statistically significant difference(P＜0.05).The low and high Cy groups showed lower relative expression of MMP-13 protein than IL-1β group,while higher relative expression of ACAN and COL2a1 protein than IL-1β group,with statistically significant difference(P＜0.01).Conclusion:Cy can improve IL-1β-induced SW1353 osteoarthritis,possibly by inhibiting SNHG14 expression.关键词
骨关节炎/木犀草苷/SW1353细胞/白细胞介素-1β/lncRNA SNHG14Key words
osteoarthritis/Cynaroside/SW1353 cell/IL-1β/lncRNA SNHG14分类
中医学引用本文复制引用
王晓戈,张靖笛,李月,田林强..木犀草苷抑制SNHG14表达改善SW1353骨关节炎细胞模型的实验研究[J].中医药导报,2023,29(11):1-5,5.基金项目
河南省重点研发计划资助项目(221111310100) （221111310100）
