中医药导报2023，Vol.29Issue(11)：6-10,5.DOI:10.13862/j.cn43-1446/r.2023.11.002
苗药吉祥草配伍余甘子抗Lewis肺癌细胞增殖组分的筛选
Screening of Anti-Lewis Lung Cancer Cells Proliferation Components of Miao Medicine Reineckia Carnea Combined with Phyllanthus Emblica
摘要
Abstract
Objective:To explore the effective material of Miao medicine Reineckia carnea combined with Phyllanthus emblica for the treatment of non-small cell lung cancer in order to provide further experimental evidence for the research of the development of anti-tumor agents.Methods:Fresh juice,traditional medicine decoction,and percolation extraction method prepared different extracts of Reineckea carnea compatibility of Phyllanthus emblica.The best extraction method of medicinal materials was selected by using Lewis lung cancer cell in vitro experimental model as evaluation method.The extracts of the best extraction method were segmented to obtain different molecular weight interval components through the membrane separation technology.Then in vitro experimental model of Lewis lung cancer cells was applied to obtain the optimal molecular weight interval.Results:The best extraction method of Reineckia carnea compatibility with Phyllanthus emblica is 95％ethanol osmoticization.The best molecular weight interval components of 95％ethanol percolation extract are 10-30 KD and ≤ 1 KD components.Conclusion:Miao medicine Reineckia carnea combined with Phyllanthus emblica can inhibit the proliferation of Lewis lung cancer cells.And the optimal components for inhibitory effect are compo-nents with a molecular weight of 10-30 KD and a molecular weight of ≤1 KD,which are permeated with 95％ethanol.关键词
肺癌/Lewis肺癌细胞/吉祥草/余甘子/渗漉/膜分离技术/苗药Key words
lung cancer/Lewis lung cancer cells/Reineckia carnea/Phyllanthus emblica/percolation extraction/membrane separation technique/Miao medicine分类
中医学引用本文复制引用
黄兴文,刘炜..苗药吉祥草配伍余甘子抗Lewis肺癌细胞增殖组分的筛选[J].中医药导报,2023,29(11):6-10,5.基金项目
国家自然科学基金项目(82160952) （82160952）
