中医药导报2023，Vol.29Issue(11)：11-17,7.DOI:10.13862/j.cn43-1446/r.2023.11.003
黄连丸剂、黄连汤剂及效应物小檗碱对2型糖尿病大鼠糖脂代谢的影响
The Effects of Huanglian Pill(黄连丸剂),Huanglian Decoction(黄连汤剂)and Effector Substance Berberine on Glucose and Lipid Metabolism in Rats with Type 2 Diabetes
摘要
Abstract
Objective:To observe the effects of Huanglian pill(HL-P),Huanglian decoction(HL-D)and berberine on glucose and lipid metabolism of diabetic rats.Methods:Totally six rats were randomly selected as control group,and 40 rats were given high fat diet and injected with streptozotocin(STZ)into the tail vein to establish diabetic rat model.Totally 30 diabetic rats were successfully modeled and randomly divided into model group,Berberine high dose group,Berberine low dose group,HL-P group and HL-D group,with six rats in each group.Berberine high dose and low dose groups were given high dose[156 mg/(kg·d)]and low dose[78 mg/(kg·d)]berberine suspension by gavage respectively.The rats in HL-P group were given Coptis powder suspension[0.64 mU(kg·d)]by gavage.The rats in HL-D group were given Coptis decoction concentrate[0.62 mU(kg·d)]by intragastric administration.Orbital blood was collected at 0,9,18,27,36,45,54 and 63 days after administration,then fasting blood glucose(FBG)and fasting insulin(FINS)were detected.And insulin sensitivity index(ISI)was calculated.Oral glucose tolerance test(OGTT)and blood lipid levels were measured by the kit on the 63rd day.Results:HPLC showed berberine is found in Coptis pills and Coptis decoction.The content of berberine in the simulated gastric fluid of Coptis pills and Coptis decoction was respectively 66.8 mg/g and 38.3 mg/g.The OGTT,FBG,FINS and blood lipid levels of diabetic rats were improved in HL-P group,HL-D group and berberine high-dose group(P＜0.05),but the OGTT,FBG,FINS and blood lipid levels of diabetic rats were not improved in berberine low-dose group(P＞0.05).Compared with HL-D group and berberine high-dose group,OGTT,FBG,FINS and ISI were improved more significantly in HL-P group at the end of treatment(P＜0.05),while there was no significant difference in the improvement of blood lipid levels in diabetic rats among treatment groups(P＞0.05).In addition,from the 45th day,the HL-P group showed better improvement of FBG level than HL-D group and berberine high-dose group(P＜0.05).From the 63rd day,HL-P group showed lower FINS level than HL-D group and berberine high-dose group(P＜0.05).The HL-P group showed higher ISI level than HL-D group from the 45th day(P＜0.05),and higher than Berberine high-dose group from the 54th day(P＜0.05).Conclusion:HL-P,HL-D and high-dose berberine played a significant role on the amelioration of blood glucose,insulin sensitivity and lipid of diabetic rats.HL-P exerted superior therapeutic effect on hypoglycemic activity and improvement of insulin resistance,compared to HL-D and high-dose berberine.关键词
2型糖尿病/黄连丸剂/黄连汤剂/小檗碱/血糖/血脂/大鼠Key words
type 2 diabetes/Huanglian pill/Huanglian decoction/berberine/blood glucose/blood lipid/rat分类
中医学引用本文复制引用
姜淑君,邹欣,董慧,李井彬,周俪姗,陆付耳,魏世超,徐丽君..黄连丸剂、黄连汤剂及效应物小檗碱对2型糖尿病大鼠糖脂代谢的影响[J].中医药导报,2023,29(11):11-17,7.基金项目
国家自然科学基金项目(81473448) （81473448）
