中医药导报2023，Vol.29Issue(11)：18-23,6.DOI:10.13862/j.cn43-1446/r.2023.11.004
"大推天河水"不同操作时长对发热幼兔的退热效果及下丘脑5-HT、NE的影响
Antipyretic Effect of Different Operation Duration of"Large Tui-Pushing Tianheshui"(大推天河水)on Young Rabbits with Fever and the Effects on 5-HT and NE in Hypothalamus
摘要
Abstract
Objective:To observe the antipyretic effect of different operation duration of"Large Tui-pushing Tianheshui"on endotoxin-induced fever of young rabbits and the influence of 5-HT and NE neurotransmitters in hypothalamus,and explore the effect relationship between massage operation duration and antipyretic effect.Methods:Totally 40 New Zealand young rabbits were randomly divided into normal group,model group,Large Tui-pushing Tianheshui 5 min group,Large Tui-pushing Tianheshui 10 min group and an ibuprofen group,with 8 rabbits in each group.Rabbits in the model group,Large Tui-pushing Tianheshui 5 min group,Large Tui-pushing Tianheshui 10 min group and ibuprofen group were subjected to prepare the endotoxin-induced fever model through the intravenous lipopolysaccharide(200 ng/mL)from the ear marginal vein,1 mUkg."Large Tui-pushing Tianheshui"manipulation was performed on the middle of the left forelimb of the young rabbits 1.5 h after modeling in the Large Tui-pushing Tianheshui 5 min group,for 5 minutes with a frequency of 50-100 times/min."Large Tui-pushing Tianheshui"manipulation was performed on the middle of the left forelimb of the young rabbits 1.5 and 2.5 h after modeling in Large Tui-pushing Tianheshui 10 min group.The duration of each session was 5 minutes,and the cumulative duration was 10 minutes.The frequency was 50-100 times/min.Ibuprofen group was given ibuprofen suspension(0.01 g/mL,1 mUkg)by gavage at 1.5 h after modeling.The dynamic changes of body temperature of young rabbits after modeling were observed,and the time point when body temperature dropped to normal was compared.The contents of 5-HT and NE in hypothalamus were determined by enzyme-linked immunoadsorption(ELISA).Results:The height of body temperature rise in Large Tui-pushing Tianheshui 5 min group was significantly lower than that in model group at 2.0-3.0 h after modeling(P＜0.01).The height of body temperature rise in Large Tui-pushing Tianheshui 10 min group was lower than that of the model group at 1.5-3.5 h after modeling(P＜0.05).The body temperature rise in ibuprofen group was lower than that in the model group at 2.0-3.5 h and 5.0-5.5 h after modeling(P＜0.01).The rise of body temperature of feverish young rabbits in Large Tui-pushing Tianheshui 10 min group was significantly lower than that in Large Tui-pushing Tianheshui 5 min group at 2.5-4.0 h after modeling(P＜0.01).The body temperature rise in ibuprofen group was lower than that in Large Tui-pushing Tianheshui 5 min group at 3.0-4.0 h and 5.5-6.0 h after modeling(P＜0.05).The temperature rise in ibuprofen group was lower than that in Large Tui-pushing Tianheshui 10 min group at 5.5-6.0 h after modeling(P＜0.05).The Large Tui-pushing Tianheshui 10 min group and ibuprofen group showed shorter antipyretic time than model group and Large Tui-pushing Tianheshui 5 min group(P＜0.01).The model group showed lower content of 5-HT in hypothalamus than normal group(P＜0.01),while higher content of NE than normal group(P＜0.01).The Large Tui-pushing Tianheshui 10 min group and ibuprofen group showed higher content of 5-HT in hypothalamus than model group(P＜0.01),while lower content of NE than model group(P＜0.01).There was no significant difference in the contents of 5-HT and NE in hypothalamus between Large Tui-pushing Tianheshui 5 min group and model group(P＞0.05).The Large Tui-pushing Tianheshui 10 min group and ibuprofen group showed higher content of 5-HT in hypothalamus than Large Tui-pushing Tianheshui 5 min group,while lower content of NE than Large Tui-pushing Tianheshui 5 min group(P＜0.01).The ibuprofen group showed lower content of NE in hypothalamus than Large Tui-pushing Tianheshui 10 min group(P＜0.01).Conclusion:Large Tui-pushing Tianheshui has a significant antipyretic effect on young rabbits with fever,and the superposition of operation time is positively correlated with the therapeutic effect.Its antipyretic effect may be related to the regulation of the balance of 5-HT and NE in the the hypothalamus.关键词
发热/大推天河水/小儿推拿/时间量效/5-羟色胺/去甲肾上腺素/兔Key words
fever/Large Tui-pushing Tianheshui/infantile massage/relation of dose-time-effect/5-HT/NE/rabbit分类
中医学引用本文复制引用
王慧娟,陈碧婵,龙倩,欧玲林,钟欢,刘迈兰,叶勇,汤伟.."大推天河水"不同操作时长对发热幼兔的退热效果及下丘脑5-HT、NE的影响[J].中医药导报,2023,29(11):18-23,6.基金项目
湖南省自然科学基金(2021JJ70108) （2021JJ70108）
湖南省"十四五"第一批中医药领军人才和学科带头人培养项目(青年神农学者)(湘中医药[2022]4号) （青年神农学者）
湖南省卫生健康高层次人才医学学科青年骨干人才培养对象(湘卫函[2023]78号) （湘卫函[2023]78号）
