中医药导报2023，Vol.29Issue(11)：24-29,6.DOI:10.13862/j.cn43-1446/r.2023.11.005
地黄饮对特应性皮炎模型小鼠的药效作用研究
Pharmacological Effects of Dihuang Decoction(地黄饮)on Mice with Atopic Dermatitis
摘要
Abstract
Objective:To investigate the effects and possible mechanisms of Dihuang decoction on atopic dermatitis(AD)model mice.Methods:Totally 36 female BALB/c mice were randomly divided into blank group,model group,control group,low-dose group of Dihuang decoction(low-dose group),medium-dose group of Dihuang decoction(medium-dose group),and high-dose group of Dihuang decoction(high-dose group).The AD model was induced by repeated stimulation of the dorsal skin with 2,4-dinitrochlorobenzene(DNCB)in all groups except the blank group.The control group,low dose group,medium dose group,and high dose group were given corresponding drugs by gavage,while the blank group and model group were given saline by gavage.Inflammation scores and organ indices(thymus and spleen)were calculated in each group,and the back skin lesions were stained with HE staining and toluidine blue staining to observe the histopathology and mast cell changes.The expression of total immunoglobulin E(IgE),interferon-γ(IFN-γ)and interleukin-4(IL-4)in the serum was detected by ELISA,and correlation analysis was carried out between the expressions of IgE,IFN-γ and IL-4 and the inflammation scores in the back skin lesions.Results:Compared with the blank group,mice in the model group showed edema,erosion,dryness or moss-like changes in the appearance of the skin on the back,and HE staining showed that the skin on the back of the mice was hyperkeratotic,thickened,and infiltrated by inflammatory cells.Compared with the model group,the appearance of the skin on the back of the mice in the low,medium,and high dosage groups showed improvement in edema,erosion,dryness,or moss-like changes,and the condition of hyperkeratotic,thickened,and infiltrated by inflammatory cells of the skin was alleviated.The model group showed higher skin lesion inflammation score,mast cell number,spleen index,serum IgE,and serum IL-4 than blank group(P＜0.05),while lower serum IFN-γ than blank group(P＜0.05).The low,medium and high dose groups showed lower skin lesion inflammation score,mast cell number,spleen index,serum IgE and serum IL-4 than model group(P＜0.05),while higher serum IFN-γ than model group(P＜0.05).The serum IgE and IL-4 were positively correlated with the score of inflammation degree of skin lesions(P＜0.05),while the serum IFN-γ was negatively correlated with the score of inflammation degree of skin lesions(P＜0.05).Conclusion:Dihuang decoction may play a therapeutic role by alleviating symptoms in AD mice by reducing mast cell infiltration and shifting the immune environment toward Th2-type cells.关键词
特应性皮炎/地黄饮/小鼠/作用机制Key words
atopic dermatitis/Dihuang decoction/mice/mechanism分类
中医学引用本文复制引用
林丽,马雪宁,任宇坤,杨素清,闫景东,安月鹏..地黄饮对特应性皮炎模型小鼠的药效作用研究[J].中医药导报,2023,29(11):24-29,6.基金项目
黑龙江省教育厅科学技术研究项目(12511522) （12511522）
哈尔滨市科技创新人才研究专项资金项目(2011RFQYS103) （2011RFQYS103）
