中医药导报2023，Vol.29Issue(11)：30-34,5.DOI:10.13862/j.cn43-1446/r.2023.11.006
水牛角对血热出血模型大鼠的止血作用及机制
Hemostatic Effect and Mechanism of Cornu Bubali on Hemorrhage Model of Blood Heat Syndrome in Rats
摘要
Abstract
Objective:To investigate the hemostatic effect and mechanism of Cornu Bubali on hemorrhage model of blood heat syndrome in rats.Methods:Totally 60 SD rats were randomly divided into normal control group,hemorrhage model group,Yunnan Baiyao(云南白药)group,Cornu Bubali high-dose group,Cornu Bubali medium-dose group and Cornu Bubali low-dose group,with 10 rats in each group.Except the normal control group,the other groups were given the dried ginger decoction combined with 5％ethanol to create hemorrhage model of blood heat syndrome.After 6 hours of modeling,rats were given Yunnan Baiyao solution(0.5 g/kg)by gav-age in Yunnan Baiyao group,and high(36 g/kg),medium(18 g/kg)and low dose(9 g/kg)Cornu Bubali decoc-tion respectively in Cornu Bubali high-dose,medium-dose and low-dose groups.Normal control group and hemorrhage model group were given equal volume of normal saline,once a day for 14 consecutive days.Body mass,water intake,food intake,urine volume,fecal water content,body mass growth rate and anal temperature were measured 1 hour after the last administration.Prothrombin time(PT),activated partial thromboplastin time(APTT),fibrinogen(FIB),thrombin time(TT),fibrin degradation product(FDP)and antithrombin(AT)were detected.The histopathological changes of lung and stomach were observed by HE staining.Results:Hemorrhage model group showed higher anal temperature than normal control group(P＜0.01),while lower growth rate of body mass,food intake,water intake,urine volume,fecal volume and fecal water content than those normal control group(P＜0.01).Yunnan Baiyao group showed lower anal temperature than hemorrhage model group(P＜0.05),while higher growth rate of body mass than hemorrhage model group(P＜0.01).Cornu Bubali high-dose and medium-dose groups showed lower anal temperature than hemorrhage model group(P＜0.01 or P＜0.05),while higher body mass growth rate,food intake,water intake,urine volume,fecal volume and fecal water content than hemorrhage model group(P＜0.01 or P＜0.05).Hemorrhage model group showed lower PT and APTT than normal control group,while higher FIB and TT than normal control group,with statistically significant differences(P＜0.01 or P＜0.05).Yunnan Baiyao group showed lower TT and FIB than hemorrhage model group(P＜0.05).Cornu Bubali high-dose and medium-dose groups showed higher PT and APTT than hemorrhage model group,while lower FIB and TT than hemorrhage model group,with statistically significant differences(P＜0.01 or P＜0.05).Hemorrhage model group showed higher AT and FDP than normal control group(P＜0.01).Yunnan Baiyao group showed lower FDP than hemorrhage model group(P＜0.01).Cornu Bubali high-dose and medium-dose groups showed lower AT and FDP than those hemorrhage model group(P＜0.01).In the normal control group,the lung and stomach tissues were basically normal.In the hemorrhage model group,inflammatory cell infiltration and bleeding occurred in lung and stomach tissues.Compared with hemorrhage model group,the pathological changes of lung and stomach tissues in Yunnan Baiyao group,Cornu Bubali high-dose group and Cornu Bubali medium-dose group were reduced to different degrees.The above indexes and histopathological changes were not obvious in Cornu Bubali low-dose group.Conclusion:Cornu Bubali may play a hemostatic role by regulating coagulation system,fibrinolytic system,and improving pathological changes of lung and stomach tissue.关键词
血热出血/水牛角/凝血功能/大鼠Key words
hemorrhage of blood heat syndrome/Cornu Bubali/coagulation function/rat分类
中医学引用本文复制引用
李海霞,周民,周有君,常依,王雅露..水牛角对血热出血模型大鼠的止血作用及机制[J].中医药导报,2023,29(11):30-34,5.基金项目
湖南省自然科学青年基金项目(2020JJ5280) （2020JJ5280）
湖南省卫生健康委科研项目(20200672) （20200672）
湖南省临床医疗技术创新引导项目(2021SK50514) （2021SK50514）
