中医药导报2023，Vol.29Issue(11)：42-49,8.DOI:10.13862/j.cn43-1446/r.2023.11.008
网络药理学结合体内实验研究藿香-龙胆草药对治疗鼻窦炎的机制
Mechanism of Huoxiang(Agastache Rugosus)-Longdancao(Gentian)in the Treatment of Sinusitis by Network Pharmacology Technology Combined with in Vivo Experiment
摘要
Abstract
Objective:To investigate the mechanism of Huoxiang(Agastache rugosus)-Longdancao(Gentian)in the treatment of sinusitis by network pharmacology technology and in vivo experiment.Methods:TCMSP and Swiss Target Prediction database were used to screen the active ingredients and targets of Huoxiang(Agastache rugosus)-Longdancao(Gentian).The disease targets of"sinusitis"were obtained by GeneCards,OMIM and Durgback platforms and then intersected with drug targets.The traditional Chinese medicine component-target network and protein interaction network(PPI)were constructed by String and Cytoscape 3.8.2 software.And Gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)signal pathway enrichment analysis were performed.Rat model of sinusitis was established.The levels of tumor necrosis factor-α(TNF-α),interleukin-1 β(IL-1 β),inter-leukin-17(IL-17)and interleukin-6(IL-6)were detected by ELISA.The protein expressions of TNF-α,IL-1β,IL-6,NF-κB p65 and IL-17C were detected by Western blotting.Results:A total of 10 active ingredients,163 drug targets,and 110 intersection targets of drug components and diseases were screened.The core active ingre-dients of Huoxiang(Agastache rugosus)-Longdancao(Gentian)were quercetin,gentioline and gentiopicrin.The core targets were AKT1,TP53,IL-6,VEGEA and TNF.KEGG enrichment was mainly involved in lipid and atherosclerosis pathway,IL-17 signaling pathway,TNF signaling pathway,etc.The results of ELISA showed that compared with the control group,the serum levels of TNF-α,IL-1β,IL-6 and IL-17 increased significantly in model group(P＜0.01).Compared with the model group,the serum levels of TNF-α,IL-1β,IL-6 and IL-17 decreased significantly in the agastache-gentian group and positive drug group(P＜0.05 or P＜0.01).Compared with the positive drug group,the serum levels of TNF-α,IL-1β,IL-6 and IL-17 increased in the agastagus-gentian group(P＜0.05).Western blotting results showed that compared with the control group,the protein relative expressions of TNF-α,IL-1β,NF-κB p65,IL-6 and IL-17C increased significantly in model group(P＜0.01).Compared with the model group,the protein relative expressions of TNF-α,IL-1β,IL-6 and IL-17C decreased significantly in the agastagus-gentiana group and positive drug group(P＜0.01).Compared with the positive drug group,the relative expressions of TNF-α,IL-1β,IL-6,and IL-17C proteins increased significantly in agastagus-gentiana group(P＜0.01),while the relative expression of NF-κB p65 protein decreased significantly in agastagus-gentiana group(P＜0.01).Conclusion:Huoxiang(Agastache rugosus)-Longdancao(Gentian)can inhibit the expression of inflammatory factors TNF-α,IL-1β and IL-6 by activating the NF-κB pathway of IL-17 to treat sinusitis.关键词
鼻窦炎/藿香/龙胆草/IL-17/NF-κB/网络药理学/大鼠Key words
sinusitis/Huoxiang(Agastache rugosus)/Longdancao(Gentian)/IL-17/NF-κB/pharmacology of network/rat分类
中医学引用本文复制引用
蔺婷,熊静,兰超,田道法,吴婷,周振峰..网络药理学结合体内实验研究藿香-龙胆草药对治疗鼻窦炎的机制[J].中医药导报,2023,29(11):42-49,8.基金项目
湖南省自然科学基金项目(2021JJ70052) （2021JJ70052）
湖南省中医药管理局基金项目(2021070) （2021070）
