化学工程2023，Vol.51Issue(12)：1-5,40,6.DOI:10.3969/j.issn.1005-9954.2023.12.001
固定化米根霉全细胞催化α-蒎烯环氧化反应
Catalysis of immobilised whole-cells Rhizopus oryzae in α-pinene epoxidation reaction
摘要
Abstract
In order to promote the green epoxidation of α-pinene,while reducing the reaction costs and improving the reusability of the catalysts,the feasibility of using immobilized whole-cells Rhizopus oryzae CGMCC 3.5040 to catalyze the epoxidation of α-pinene was studied in this experiment.The reaction conditions were optimized by using a single-factor experimental method.The results show that the conversion of α-pinene and the yield of 2,3-epoxypinene are(99.7±0.1)％and(95.1±1.1)％,respectively,after reacting 8 h with 150 mg of immobilized cell addition,the volume ratio 3∶1 of organic phase to 30％ H2 O2 solution and 1.5 mmol of octanoic acid.In addition,the immobilized whole-cell catalysts were reused.It is found that using toluene as a cleaning agent can effectively maintain the stability of the catalysts and the catalysts retain more than 75.0％ conversion rate after seven cycles.This study provides a new idea for an environmentally friendly catalytic approach and a new option for chemical synthesis in industry.关键词
α-蒎烯/脂肪酶/固定化细胞/α-蒎烯环氧化/重用性Key words
α-pinene/lipase/immobilized cells/epoxidation of α-pinene/reusability分类
生物科学引用本文复制引用
王宁,许丽莉,刘幽燕,唐爱星..固定化米根霉全细胞催化α-蒎烯环氧化反应[J].化学工程,2023,51(12):1-5,40,6.基金项目
国家自然科学基金资助项目(21966007) （21966007）
