四川大学学报（医学版）2023，Vol.54Issue(6)：1219-1226,8.DOI:10.12182/20231160601
急性敌草快中毒患者的血浆代谢组学特征初探
A Preliminary Study on the Plasma Metabolomic Profiles of Patients Suffering From Acute Diquat Poisoning
摘要
Abstract
Objective To analyze the plasma metabolomic features of patients suffering from acute diquat(DQ)poisoning and to explore the molecular mechanism and potential biomarkers of DQ poisoning.Methods A total of 7 patients suffering from acute DQ poisoning were enrolled in the DQ poisoning group.The poisoning of these patients occurred within a 12-h window at the time of enrollment.Meanwhile,7 healthy immediate family members of the patients were enrolled as the normal controls.Liquid chromatography-mass spectrometry(LC-MS)was used to perform non-targeted metabolomic profiling of the plasma samples and to screen and identify differential metabolites and metabolic pathways.Results A total of 104 metabolites were screened and identified(P＜0.05 and the variable importance in the projection[VIP]＞1).Compared with those of the control group,61 metabolites,such as sorbitol and galactitol,were up-regulated,and 43 metabolites,such as myo-inositol and gamma-glutamylcysteine,were down-regulated in the DQ poisoning group.Pathway enrichment analysis revealed changes in 11 metabolic pathways,including those for galactose metabolism and linoleic acid metabolism(P＜0.05).Conclusion Metabolomics analysis of plasma samples from DQ poisoning patients shows that DQ mainly interferes with the metabolism of energy,amino acids,and lipids,thus causing metabolic disorders.Some potential biomarkers closely associated with oxidative stress and organ damage of the liver,kidney,and nervous system have been identified.关键词
敌草快/中毒/代谢组学/血浆Key words
Diquat/Poisoning/Metabolomics/Plasma引用本文复制引用
胡惠,杜宇..急性敌草快中毒患者的血浆代谢组学特征初探[J].四川大学学报（医学版）,2023,54(6):1219-1226,8.基金项目
四川省科技厅科研课题基金(No.23ZDYF1888)资助 （No.23ZDYF1888）
