海洋科学2023，Vol.47Issue(10)：32-42,11.DOI:10.11759/hykx20230412001
盐度胁迫下凡纳滨对虾体内虾青素在着色与抗氧化的资源权衡
Resource tradeoff between coloring and antioxidation of astaxanthin in Litopenaeus vannamei under salinity stress
摘要
Abstract
The environmental pressure generated by industrial culture causes stress and weakening of the body color of the shrimp.Our results show that astaxanthin(d-AST)and synthetic astaxanthin(p-AST)contents in the hepa-topancreas of shrimps treated with astaxanthin increased by 48.0%and 17.5%,respectively,compared with those who were not treated with any form of astaxanthin.Astaxanthin content in shrimp shells increased by 42.8%and 45.2%,respectively.d-AST rich in esterified astaxanthin was deposited in high amounts in the hepatopancreas,whereas p-AST composed of free astaxanthin was deposited in high amounts in the shell.Different tissues had preferences for different forms of astaxanthin.The body color of shrimps in the two treatment groups was signifi-cantly enhanced.Astaxanthin content in hepatopancreas of d-AST and p-AST decreased by 15.1%and 5.7%after applying salinity stress,respectively,whereas its content in the control group remained unchanged.Astaxanthin content in shrimp shells of d-AST,p-AST and control group significantly decreased by 17.8%,52.9%,and 14.3%,respectively.The expression of the gene encoding the astaxanthin transporter β-1,3-glucan-binding protein(βGBP-HDL)was significantly upregulated after 24 h of stress,which may reduce astaxanthin content in the shrimp shell.A simultaneous upregulation in the expression of antioxidant genes with the progression of stress was ob-served in the two treatment groups compared with the control group.The antioxidant capacity of the groups in-creased,subsequently decreased,and finally reached the maximum value at 48 h.At 48 h,d-AST and p-AST were 1.35 and 1.30 times that of the control group,respectively.Therefore,we speculate that astaxanthin content in shrimp affects pigmentation and antioxidant functions according to the resource balance principle.Under environ-mental stress conditions,astaxanthin used for pigmentation(shrimp shell)is preferentially transported to the hepa-topancreas to participate in antioxidation,which weakens body color and increases antioxidant capacity.关键词
凡纳滨对虾(Litopenaeus vannamei)/虾青素/体色/抗氧化Key words
Litopenaeus vannamei/astaxanthin/body color/antioxidation分类
农业科技引用本文复制引用
杨梦煊,王宝杰,刘梅,蒋克勇,仲晨,王雷..盐度胁迫下凡纳滨对虾体内虾青素在着色与抗氧化的资源权衡[J].海洋科学,2023,47(10):32-42,11.基金项目
国家自然科学基金项目(NSFC-山东联合基金)(U1706209) （NSFC-山东联合基金）
黄河三角洲产业领军人才项目 （）
山东省海洋科学研究院科研补助经费National Natural Science Foundation of China-Joint 322 Fund of Shandong People's Government,No.U1706209 （）
Yellow River Delta Industry Leading Talent Project （）
Research Supplement Fund for Marine Science Research Institute of Shandong Province （）
