海洋科学2023，Vol.47Issue(10)：94-111,18.DOI:10.11759/hykx20220819003
光谱对许氏平鲉消化代谢节律及生理应激研究
Effects of spectra on digestion,metabolic rhythm,and physi-ological stress of Sebastes schlegelii
摘要
Abstract
The serum,intestinal tract,and liver of Sebastes schlegelii were sampled over a period of 24 h(8:00,12:00,16:00,20:00,and 24:00)under five spectra(red,green,yellow,blue,and white lights,respectively;photoperiod 12L:12D).to investigate the digestion and metabolism levels and peripheral hormone rhythms.Results revealed that the digestion and metabolism enzymes of Sebastes schlegelii under different spectra exhibited rhythmic patterns,except for trypsin.The peak phases of α-amylase(α-AMS)and lipase(LPS)under yellow light shifted to the left compared to those under white light,and trypsin(TRY)showed no temporal difference.The peak phase of Lactate dehydrogenase(LDH)under green,yellow,and red lights moved to the left compared to that under white light.In the five spectral environments,red and yellow light affected the peak of digestive enzyme activity earlier.The peak phase of pyruvate kinase(PK)shifted to the left under green,blue,and red lights.The secretion of hexokinase(HK)was observed under green,blue,and yellow lights,and its peak phase shifted to the left compared to that under white light.In five spectral environments,green and blue lights affected the peak of metabolic enzymes substantially.The melatonin level in the serum of Sebastes schlegelii was low during the day and high at night.Moreover,cortisol decreased during the day and began to diminish continuously after reaching its highest value at midnight.Changes in the peak phase indicate variations in circadian rhythms.The above results show that different spectra can influence the physiological meta-bolic rhythm of fish.Thus,future research should fully consider the biological effect of spectra on cultured organisms and then formulate appropriate lighting conditions.关键词
许氏平鲉/光谱/昼夜节律/消化代谢/应激Key words
Sebastes schlegelii/spectra/circadian rhythm/digestion and metabolism/stress分类
农业科技引用本文复制引用
房莹莹,陈璐璐,刘松涛,孙飞,吴燕玲,李鑫,刘鹰,马贺..光谱对许氏平鲉消化代谢节律及生理应激研究[J].海洋科学,2023,47(10):94-111,18.基金项目
国家自然基金(32202961) （32202961）
辽宁省科学技术计划项目(2021JH2/10200011) （2021JH2/10200011）
设施渔业教育重点实验开放课题(202202) （202202）
现代农业产业技术体系专项资金(CARS-49)National Natural Science Foundation of China,No.32202961 （CARS-49）
Science&Technology Program of Liaoning Province,No.2021JH2/10200011 （）
Open Project of Key Laboratory of Environment Controlled Aquaculture(Dalian Ocean University)Ministry of Education,No.202202 （Dalian Ocean University）
Modern Agro-industry Technology Research System,No.CARS-49 （）
