华中师范大学学报（自然科学版）2023，Vol.57Issue(6)：878-889,12.DOI:10.19603/j.cnki.1000-1190.2023.06.013
瓦尼桑黄核苷类成分的液体发酵工艺优化及其细胞毒活性
Optimization of liquid fermentation and cytotoxic activity of nucleosides from Sanghuangporus vaninii
摘要
Abstract
Sanghuangporus vaninii is a valuable medicinal fungus,which contains nucleoside components that can be developed into pharmaceuticals and health products with antiviral,antitumor and antibacterial activities.In this paper,the fermentation conditions and ratio of carbon,nitrogen and inorganic salts for liquid fermentation of Sanghuangporus vaninii were optimized by single factor and orthogonal tests,using nucleoside yield as an indicator.And the effects of adding different precursors and regulatory factor on the yield of nucleoside were investigated.One of the key factors affecting nucleoside production was screened out as exogenous precursors such as hypoxanthine and adenine.The optimal fermentation conditions were determined as follows:10％inoculum,initial pH 7,incubation temperature 32 ℃,shaker speeds 140 r·min-1 and incubation time 5 d.The optimal medium ratios were as follows:glucose 15.00 g·L-1,sucrose 20.00 g·L-1,peptone 5.00 g·L-1,beef paste 2.50 g·L-1,MgSO4 1.00 g·L-1,CaCl2 1.50 g·L-1,ZnSO4 1.00 g·L-1,MnSO40.50 g·L-1.Optimal amount of precursor and regulatory factor were hypoxanthine 1.50 g·L-1,adenine 2.50 g·L-1,sodium pyruvate 3.50 g·L-1 and quercetin 0.25 g·L-1.Under the optimum conditions,the nucleoside yield of liquid fermentation of Sanghuangporus vaninii was 291.16±6.21 mg·L-1,32.3 times higher than that before optimization.In addition,the cytotoxic activities of the nucleoside enriched products from the fruiting body and sample that before and after optimization were compared.The experimental results showed that the optimized liquid fermentation nucleoside products had the best inhibitory activity on human hepatoma cell line HepG2,with a higher inhibitory rate than the enriched products before optimization and the fruiting body products,This paper provided a theoretical basis for the further development and utilization of Sanghuangporus vaninii.关键词
瓦尼桑黄/核苷/正交实验/培养基优化/前体/调控因子Key words
Sanghuangporus vaninii/nucleoside/orthogonal experiment/optimization of culture medium/precursors/regulatory factor分类
医药卫生引用本文复制引用
祝梓旋,王倩,刘呈雄,刘朝霞,张诗浩,田兴,李雪妍,邹坤,尉小琴,李啸,曾建红,马明华..瓦尼桑黄核苷类成分的液体发酵工艺优化及其细胞毒活性[J].华中师范大学学报（自然科学版）,2023,57(6):878-889,12.基金项目
国家自然科学基金项目(82204237) （82204237）
天然产物研究与利用湖北省重点实验室开放基金项目(2022NPRD01). （2022NPRD01）
