家畜生态学报2023，Vol.44Issue(12)：21-24,4.DOI:10.3969/j.issn.1673-1182.2023.12.004
猪源多重耐药化脓隐秘杆菌全基因组测序分析
Whole Genome Sequencing of Multidrug Resistant Trueperella pyogenes from Pigs
摘要
Abstract
Trueperella pyogenes is a common opportunistic pathogen in the economic animal breeding.Combined with domestic and overseas research data,it was found that Trueperella pyogenes have acquired a great resistance because of the overuse of antibiotics.The purpose of this research was to study the mech-anism of drug-resistance of Trueperella pyogenes isolated in Jilin.The dominant strains were isolated from the lungs of a suffering sow in Jilin.The strains were identified as Trueperella pyogenes and named TP11 through PCR amplification of 16S rRNA gene and its MIC was determined by agar plate dilution method.The mechanism of drug resistance was studied through the third generation whole genome sequencing and the results of drug sensitivity test were found consistent with the drug resistant genetypes from sequen-cing.It is hoped to provide some reference data for the new prophylaxis and treatment and the research and development of the newtype drug and drug resistance inhibitor of Trueperella pyogenes bacillosis.关键词
化脓隐秘杆菌/全基因组测序/耐药机制/猪Key words
Trueperella pyogenes/whole genome sequencing/drug resistance mechanism/pig分类
农业科技引用本文复制引用
张涵,卢殿杰,庄科勤,徐菁岐,孟雨晴,马威,董文龙..猪源多重耐药化脓隐秘杆菌全基因组测序分析[J].家畜生态学报,2023,44(12):21-24,4.基金项目
吉林省教育厅科学技术研究项目(JJKH20210423KJ) （JJKH20210423KJ）
