家畜生态学报2023，Vol.44Issue(12)：69-73,5.DOI:10.3969/j.issn.1673-1182.2023.12.012
呼和浩特市周边地区规模化牛场大肠杆菌K99流行情况调查
Investigation on the Prevalence of Escherichia coli K99 in Large-scale Cattle Farms around Hohhot
摘要
Abstract
Calf diarrhea caused by Escherichia coli K99 has caused huge economic losses to the cattle industry.In order to investigate the prevalence of K99,380 fecal and blood samples were randomly collect-ed from 7 large-scale cattle farms around Hohhot from August 2020 to July 2021.The prevalence of K99 antigen and antibody in this area was investigated by PCR and ELISA.PCR positive samples were isolated and cultured.K99 isolate was identified by morphology,biochemical test,pili specific gene amplification and sequencing,and the drug sensitivity was further evaluated by drug sensitivity test,so as to provide reference basis for clinical rational application of antibiotics and formulation of prevention and treatment measures.The results showed that 25 out of 380 fecal samples were K99 positive by PCR,and the positive rate was 6.58％.Among 380 blood samples,99 positive blood samples were detected by ELISA,and the positive rate was 26％.Most of them were observed in 0～2 months old calves,and the positive rate was 48.28％.Eight strains of K99 were identified by PCR sequencing of pili gene.The drug sensitivity results showed that the isolates were sensitive to gentamicin,ofloxacin,streptomycin,amikacin and kanamycin.This study indicates that K99 infection exists in calves with diarrhea in the surrounding areas of Hohhot.The most common cases were 0 to 2 months of age.It provides reference for the farmers to appropriately use medicine clinically and to prevent the disease.关键词
牛腹泻/大肠杆菌K99/PCR/ELISAKey words
cattle diarrhea/Escherichia coli K99/PCR/ELISA分类
农业科技引用本文复制引用
刘平平,谢梦圆,李文豪,郝彦儒,王建龙,王旭红,徐晓静..呼和浩特市周边地区规模化牛场大肠杆菌K99流行情况调查[J].家畜生态学报,2023,44(12):69-73,5.基金项目
内蒙古自治区科技重大专项(2020ZD0006) （2020ZD0006）
