解放军医学杂志2023，Vol.48Issue(12)：1387-1394,8.DOI:10.11855/j.issn.0577-7402.2414.2023.0327
血必净注射液抑制肺血管内皮糖萼降解改善重症中暑大鼠急性肺损伤
Xuebijing injection inhibits the degradation of pulmonary vascular endothelial calyx to improve acute lung injury induced by severe heatstroke
摘要
Abstract
Objective To investigate the possible mechanism of Xuebijing injection in regulating abnormal degradation of pulmonary vascular endothelial calyx to improve acute lung injury induced by severe heatstroke.Methods Forty-eight Wistar rats were randomly divided into control group,heatstroke group and Xuebijing group.Before heatstroke induction,rats in Xuebijing group were administrated with Xuebijing injection(2 ml/kg,2 times/d)for 3 days.All rats were exposed to an environment with temperature of(40±2)℃and humidity of 65%±5%for 60 minutes to induce heatstroke.Two hours later,the lung wet/dry weight ratio was recorded;the concentration of proteins in BALF was measured;the pulmonary vascular permeability was measured by Evans blue(EB);HE staining was used to observe the pathological changes of lung tissue;the changes of hyaluronic acid(HA)on the surface of pulmonary vessels were observed by immunofluorescence;Western blotting was used to detect the expression of Syndecan-1,Glypican-1,VE-Cadherin,Occludin,VCAM-1 and E-selectin in lung tissues;Enzyme-linked immunosorbent assay(ELISA)was utilized to quantify the concentration of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)in serum and heparanase(HPA)in lung tissue.Results Xuebijing could decrease the lung wet/dry weight ratio,reduce protein exudation and improve pulmonary vascular permeability(P<0.01);reduce the histological injury(P<0.01);reduce the degradation of HA,Syndecan-1 and Glypican-1 on the surface of pulmonary vessels(P<0.01);increase the expression of VE-Cadherin and Occludin(P<0.01);regulate the overexpression of VCAM-1 and E-selectin(P<0.01);down-regulate the expression of TNF-α,IL-6 and HPA(P<0.01).Conclusion Xuebijing injection decrease the expression of HPA,improve the disintegration of pulmonary vascular endothelial calyx,repair the integrity of pulmonary vessels,reduce the damage of cell connections,down-regulate the expression of adhesion molecules,inhibit the inflammatory reaction,relieving acute lung injury caused by severe heat stroke.关键词
重症中暑/血必净注射液/糖萼/急性肺损伤Key words
severe heatstroke/Xuebijing injection/glycocalyx/acute lung injury分类
医药卫生引用本文复制引用
丁程佳,陀劲,曹晶晶,黄洁恩,刘超帆,童华生,陈怿..血必净注射液抑制肺血管内皮糖萼降解改善重症中暑大鼠急性肺损伤[J].解放军医学杂志,2023,48(12):1387-1394,8.基金项目
This work was supported by the Natural Science Foundation of Guangdong Province(2019A1515011777),the Project of Administration of Traditional Chinese Medicine of Guangdong Province(20190320121131),and the Dongguan Binhaiwan Central Hospital High Level Scientific Research Fund(2022007) 广东省自然科学基金(2019A1515011777) （2019A1515011777）
广东省中医药局科学技术研究基金(20190320121131) （20190320121131）
东莞市滨海湾中心医院高水平科研孵化基金(2022007) （2022007）
