井冈山大学学报（自然科学版）2023，Vol.44Issue(6)：53-60,8.DOI:10.3969/j.issn.1674-8085.2023.06.007
基于转录组测序技术揭示克罗恩病中相关的可变剪接事件
TRANSCRIPTOME ANALYSIS OF ALTERNATIVE SPLICING EVENTS IN CROHN'S DISEASE
摘要
Abstract
Transcriptome sequencing analysis was used to understand the alternative splicing events of Crohn's disease(CD),and to identify highly associated RNA binding proteins and potential regulatory genes,so as to reveal the genetic regulatory mechanisms during CD formation.We downloaded and preprocessed 33 published transcriptome sequencing data(GSE66207 RNA-seq)of colon tissues from CD patients with three different disease behaviors,including 6 nonstricturing and nonpe-netrating(B1),6 stricturing(B2),and 8 penetrating/fistulizing(B3),and that of 13 non-IBD controls(Nor).We performed transcriptome analysis of differentially expressed genes and alternative splicing events,and correlation analysis between the differentially expressed RBP and differential alternative splicing genes associated with CD.Results show:(1)Analysis of the number of differentially expressed genes found that more B1 genes were down-regulated,and more B2 and B3 genes were up-regulated.The number of differentially expressed genes in B3 is larger than that of B2,suggesting that more genes are differentially expressed after the disease progressing into penetration.(2)A large number of abnormal alternative splicing occur in DNA replication and cell cycle progression in CD.GO analysis showed that the abnormal alternative splicing genes involved in cell growth in B1,B2 and B3,and obvious apoptosis-related pathways were found in B2 and B3.(3)Fourteen RNA-binging proteins(DAZL,DDX3Y,RPS4Y1,EIF1AY,TDRD1,RBM24,RNASE2,APOBEC1,NXF3,EEF1A2,RBFOX3,PALYL,RNF17,and OASL)were identified as involved in the regulation of alternative splicing of related genes in CD.In this study,we performed gene alternative splicing analysis of three phenotype CD,and found that a number of genes involved in cell proliferation,apoptosis and key signaling pathways undergo abnormal splicing regulated by 14 RNA binding proteins in CD progression.关键词
转录组分析/克罗恩病/可变剪接/RNA结合蛋白Key words
transcriptome analysis/Crohn's disease/alternative splicing/RNA-binding proteins分类
生物科学引用本文复制引用
王芬芬,郭丽,彭成明,李军,温志立..基于转录组测序技术揭示克罗恩病中相关的可变剪接事件[J].井冈山大学学报（自然科学版）,2023,44(6):53-60,8.基金项目
国家自然科学基金面上项目(82070594) （82070594）
江西省自然科学基金青年项目(20224BAB216020) （20224BAB216020）
