荆楚理工学院学报2023，Vol.38Issue(6)：12-21,10.
复方石斛总黄酮提取及活性研究
Study on Extraction and Activity of Total Flavonoids from Compound Dendrobium Officinale
摘要
Abstract
Purpose:The total flavonoids were extracted from compound Dendrobium officinale(Dendrobium officinale-Codonopsis pilosula-Huangqi-Huangjing = 2:3:3:2).Method:The extraction process was optimized through single factor ex-periments and response surface methodology,and the antioxidant activity in vitro and hypoglycemic activity in vivo of the extracts were measured.Results:The results showed that the optimal extraction process conditions for total flavonoids in compound Dendro-bium officinale were ethanol concentration of 60%,ultrasound time of 50 minutes,liquid-solid ratio of 35,and total flavonoid ex-traction rate of 2.59%.The half inhibition rates of 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH),2,2-biazo-bis-3-ethylben-zothiazoline-6-sulfonic acid(ABTS+),and hydroxyl radicals were determined to be 7.189 μg/ml,1.288 μg/ml,and 1.468 μg/ml,respectively.The nematode experiment was used to determine its hypoglycemic activity in vivo,and when the extract was prepared into a 120 mg/ml solution,it had good hypoglycemic activity.Conclusion:The compound dendrobium extract prepared in the study has good antioxidant and hypoglycemic effects.关键词
复方石斛/总黄酮/响应面法/抗氧化活性/降糖活性Key words
Compound Dendrobium officinale/Total flavonoids/Response surface methodology/Antioxidant activity/hypo-glycemic activity分类
医药卫生引用本文复制引用
马丹,尚书游,陈欣雨,付佳欣,阮军..复方石斛总黄酮提取及活性研究[J].荆楚理工学院学报,2023,38(6):12-21,10.基金项目
湖北省教育厅支持企业技术创新发展项目(2021BLB211) （2021BLB211）
湖北省教育厅科学研究计划项目(B2018233) （B2018233）
荆楚理工学院科学研究项目(HX20230023) （HX20230023）
湖北省科技厅科学研究项目(2022BEC013) （2022BEC013）
