解剖学杂志2023，Vol.46Issue(5)：390-394,5.DOI:10.3969/j.issn.1001-1633.2023.05.005
核苷(酸)类似物联合聚乙二醇干扰素对慢性乙型肝炎大鼠法尼醇X受体-成纤维细胞生长因子19通路、枯否细胞极化的影响
Effects of nucleoside acid analogues combined with pegylated interferon on farnesol X receptor-fibroblast growth factor 19 pathway and Kupffer cell polarization in chronic hepatitis B rats
摘要
Abstract
Objective:To investigate the effects of nucleoside(acid)analogues combined with pegylated interferon on farnesol X receptor(FXR)-fibroblast growth factor 19(FGF19)pathway and Kupffer cell polarization in chronic hepatitis B rats.Methods:SPF SD male rats were randomly divided into normal group,model group,nucleoside(acid)analogue(nucleotide)group,pegylated interferon(interferon)group,and combination group.Except for the normal group,chronic hepatitis B was modeled by intraperitoneal injection of hepatitis B virus.After successful modeling,the nucleotide group was given 10 mg/kg lamivudine.The interferon group was subcutaneously injected with 5 μg/kg pegylated interferon,and the combined group was given lamivudine combined with pegylated interferon.The normal group and the model group were given gavage with the same volume of normal saline at the same time.Serum virus was detected by enzyme-linked immunosorbent assay,liver function was detected by automatic biochemical analyzer,and pathological morphology of liver tissue was detected by H-E staining.The expression of FXR-FGF19 pathway protein was detected by Western blotting and Kuper cell polarization was detected by immunohistochemistry.Results:Compared with the normal group,the serum HBsAg,HBeAg,ALT,AST,TBIL,iNOS and CD163 positive expression rates were significantly increased in the model group,while the protein expressions of FXR and FGF19 in liver tissue were significantly decreased.Serum HBsAg,HBeAg,ALT,AST,TBIL,iNOS and CD163 positive expression rates were significantly decreased in nucleotide group,interferon group and combination group,while FXR and FGF19 protein expressions in liver tissue were significantly increased,and the combination group showed significant reducition compared with the interferon group.Conclusion:Nucleoside(acid)analogues combined with pegylated interferon can significantly activate FXR-FGF19 pathway,inhibit Kupffer cell M1 polarization,and promote Kupffer cell M2 polarization,and have an improvement effect on rats with chronic hepatitis B.关键词
核苷(酸)类似物/聚乙二醇干扰素/慢性乙型肝炎/法尼醇X受体-成纤维细胞生长因子19通路/枯否细胞/大鼠Key words
nucleoside(acid)analogs/pegylated interferon/chronic hepatitis B/farnesol X receptor-fibroblast growth factor 19 pathways/Kupffer cell/rat引用本文复制引用
颜成果,闪海霞,吴玉卓,卢瑞杰,温泉..核苷(酸)类似物联合聚乙二醇干扰素对慢性乙型肝炎大鼠法尼醇X受体-成纤维细胞生长因子19通路、枯否细胞极化的影响[J].解剖学杂志,2023,46(5):390-394,5.基金项目
河南省医学科技公关计划联合共建项目(LGHJ20191457) （LGHJ20191457）
