解剖学杂志2023，Vol.46Issue(5)：406-410,439,6.DOI:10.3969/j.issn.1001-1633.2023.05.008
散结片对肝癌大鼠巨噬细胞极化及MAPK/JNK信号通路的作用机制
Effect of Sanjie tablet on macrophage polarization and MAPK/JNK signaling pathway in rats with liver cancer
摘要
Abstract
Objective:To explore the effect mechanism of Sanjie tablet on macrophage polarization and MAPK/JNK signaling pathway in rats with liver cancer.Methods:SPF grade male nude mice(SD strain)were randomly divided into normal group,model group,sorafenib group,and Sanjie tablet group.The number of deaths of the rats was recorded,and survival curves were plotted.HE staining was used to examine the pathological morphology of liver tissues,immunohistochemistry was used to detect the protein expression of macrophage-specific markers in liver tissues,and immunoblotting was used to detect the protein expression of MAPK/JNK signaling pathway-related proteins in liver tissues.Results:Compared with the normal group,the number of deaths,CD163,p38MAPK,and JNK protein expression in liver tissues of the model group were significantly increased,the survival curve,and CD11c protein expression in liver tissues were significantly decreased.Compared with the model group,the sorafenib group and Sanjie tablet group showed significantly reduced number of deaths,tumor mass and volume,CD163,p38MAPK,and JNK protein expression in liver tissues,and significantly increased survival curve and CD11c protein expression in liver tissues.Additionally,the Sanjie tablet group showed significant differences compared with the sorafenib group.There was no statistically significant difference in p38MAPK and JNK protein expression in liver tissues between the inhibitor group and Sanjie tablet group,while the combined group showed a significant decrease compared with the inhibitor group.Conclusion:Sanjie tablet may inhibit the tumor growth of rats with hepatocellular carcinoma by suppressing MAPK/JNK signaling pathway,inhibiting the polarization of macrophages towards M2 type and promoting the polarization towards M1 type.关键词
散结片/肝癌/巨噬细胞极化/MAPK/JNK信号通路Key words
Sanjie tablet/liver cancer/macrophage polarization/MAPK/JNK signaling pathway引用本文复制引用
张远,董晶,国滨,徐振华,时桢,李金芳..散结片对肝癌大鼠巨噬细胞极化及MAPK/JNK信号通路的作用机制[J].解剖学杂志,2023,46(5):406-410,439,6.基金项目
国家自然科学基金培育项目(19NSP012) （19NSP012）
