净水技术2023，Vol.42Issue(12)：44-51,127,9.DOI:10.15890/j.cnki.jsjs.2023.12.006
污水中新型冠状病毒富集方法研究进展
Research Progress on Concentration Methods of SARS-CoV-2 in Wastewater
郑晓雨 1鲁玺龙 2闻武 1杨帆 2赵兴春2
作者信息
- 1. 公安部禁毒情报技术中心,北京 100193||公安部物证鉴定中心,北京 100038
- 2. 公安部物证鉴定中心,北京 100038
- 折叠
摘要
Abstract
Since the outbreak of COVID-19,SARS-Coronavirus-2(SARS-CoV-2)presence in wastewater which has been increasingly reported by more and more countries plays an important role in dynamically surveilling the pandemic development trend,providing an early warning and estimating the prevalence of epidemic interventions.Due to the extremely high infectivity of SARS-CoV-2 and the relatively low copy number in wastewater,it is vital to develop applicable concentration methods for accurate qualitative monitoring and quantitative analysis.This paper reviews and evaluates the concentration methods of SARS-CoV-2 in wastewater from the perspective of important factors such as recovery,time consumption,cost and throughput.Virus adsorption-elution and flocculation precipitation methods are relatively low cost and time-consuming which are suitable for primary enrichment of samples with large volume,while ultracentrifugation and ultrafiltration methods commonly used as secondary concentration are high cost and time-saving.Optimizing the pore size of membrane filter,flocculant,centrifugation speed and nominal molecular weight limit can improve the concentration efficiency such as recovery rate and so on.What's more,future research needs including choosing appropriate alternative viruses,reducing the uncertainty of sample matrix,optimizing RNA extraction methods,and establishing a normative method are delineated for details to improve the reliability and comparability of the results.关键词
新冠肺炎病毒/污水流行病学/富集/疫情/环境样品Key words
SARS-CoV-2/wastewater-based epidemiology/concentration/epidemic/environmental sample分类
环境科学引用本文复制引用
郑晓雨,鲁玺龙,闻武,杨帆,赵兴春..污水中新型冠状病毒富集方法研究进展[J].净水技术,2023,42(12):44-51,127,9.
