净水技术2023，Vol.42Issue(12)：63-71,9.DOI:10.15890/j.cnki.jsjs.2023.12.008
住宅厨房给水管网末梢微生物多样性分析
Analysis of Microbial Diversity at the End of Water Supply Pipelines Network in Residential Kitchens
摘要
Abstract
Collecting water samples from the tap,branch pipelines network and water filter of residential kitchen water supply pipelines network,extracting genomic DNA by proteinase K cleavage method,and analyzing the microbial community structure,metabolic function,antibiotic resistance genes(ARGs)and virulence factors based on petagenomics,the safety risk of drinking water at the end of water supply branch pipelines were explored.The results indicated that there would be multiple microorganisms presented in different parts of the residential kitchen water supply network and had different specific functions,carbohydrate metabolism was the pathway with the highest number of KEGG annotations in the residential kitchen water supply network endings samples,the adeF gene was the optimal antibiotic resistance ontology(ARO)resistance gene,and the aggressive virulence factor was the main type of bacterial virulence,the end attachment of water supply branch pipelines was more convenient for microbial enrichment and had a higher risk of antibiotic resistance genetic pollution.This study can provide guidance for the study of microbial diversity at the ends of residential kitchen water supply networks,and provide new ideas for ensuring the biological safety of drinking water.关键词
给水管网/宏基因组学/微生物群落结构/抗生素抗性基因(ARGs)毒力因子Key words
water supply pipelines network/metagenomics/microbial community structure/antibiotic resistance genes(ARGs)/virulence factor分类
土木建筑引用本文复制引用
高将,张宝军,孙悦,袁涛..住宅厨房给水管网末梢微生物多样性分析[J].净水技术,2023,42(12):63-71,9.基金项目
徐州市重点研发计划项目(KC19221) （KC19221）
徐州市应用基础研究计划项目(KC19049) （KC19049）
徐州市基础研究计划青年科技人才项目(KC23036) （KC23036）
