实用心电学杂志2023，Vol.32Issue(6)：416-421,6.DOI:10.13308/j.issn.2095-9354.2023.06.007
两种不同方法制作犬心房颤动模型的安全性分析
Safety analysis of two different methods for producing canine atrial fibrillation models
摘要
Abstract
Objective To compare the safety of the thoracotomy implantation of electrodes and the implantation of electrodes under X-ray fluoroscopy in creating a rapid atrial pacing model,and to provide a basis for producing an atrial fibrillation(AF)animal model.Methods Twelve experimental dogs were randomly divided into two groups according to different pathways of pacemaker electrode implantation.In the thoracotomy group(n = 6),pacemaker electrodes were fixed in the left atrial appendage following thoracotomy while in the intervention group(n=6),pacemaker electrodes were implanted into the right atrial appendage or right atrium through the external jugular vein.Based on stable general situations,the pacemaker was opened and continuously paced for 6 weeks at 400 times per minute in each group.Changes of the cardiac structure was evaluated by performing cardiac B-ultrasound examinations separately in the baseline state and after six weeks of continuous pacing.After the surgery,subcutaneous infection,pouch infection,bloody infection,infectious endocarditis,heart failure were assessed by observing the wound,monitoring body temperature,and detecting procalcitonin and NT-proBNP.Results Among the 12 dogs,one in the thoracotomy group died on the third day after the surgery while one in the intervention group suddenly died on the seventh day after the surgery;the remaining 10 dogs all completed the experiment.AF could be induced in both groups.Compared with the cardiac structure before the pacing,the cardiac structure of both groups change to a certain extent following six weeks of pacing;the left atrial diameter,the left ventricular end-systolic diameter,the left ventricular end-diastolic diameter,the right atrial diameter,and the right ventricular diameter of the two groups all increase after six weeks of pacing.However,there is no statistically significant difference of ejection fraction before and after pacing.The incidence of heart failure in the thoracotomy group is significantly higher than that in the intervention group(50.0%vs.16.7%,P＜0.05)while the infection rate is also significantly different between the two groups(66.7%vs.33.3%,P＜0.05).Conclusion In terms of reducing the infection rate and the incidence of heart failure,the method of implanting electrodes in an external jugular vein under X-ray fluoroscopy is superior to the thoracotomy implantation of electrodes to produce a canine rapid atrial pacing model.关键词
犬/动物模型/心房刺激/心房颤动/感染/心力衰竭Key words
dog/animal model/atrial stimulation/atrial fibrillation/infection/heart failure分类
医药卫生引用本文复制引用
麦麦提阿卜杜拉·麦麦提敏,杨徐,张玲,麦麦提艾力·麦麦提敏,曹桂秋,李耀东,孙华鑫,马嵋,汤宝鹏..两种不同方法制作犬心房颤动模型的安全性分析[J].实用心电学杂志,2023,32(6):416-421,6.基金项目
新疆维吾尔自治区自然科学基金资助项目(2021D01D16,2021D01E27) （2021D01D16,2021D01E27）
