江西科学2023，Vol.41Issue(6)：1071-1076,6.DOI:10.13990/j.issn1001-3679.2023.06.008
夹竹桃天蛾胚胎细胞的原代培养
Research Advances on Primary Culture of Daphnis nerii Embronic Cell in Vitro
摘要
Abstract
Daphnis nerii(Linnaeus)is a notorious pest that has a wide geographic distribution.In this study,we employed a mechanical crushing method to establish primary cultures of Daphnis nerii eggs and determined Grace's insect medium as the most optimal culture medium.Primary culture of Daphnis nerii embryonic cells lasted up to 12 months,and various forms of primary cells were differ-entiated in the primary culture.Our result provides crucial experimental basis for establishing Daph-nis nerii cell lines in future research.关键词
夹竹桃天蛾/原代培养/形态学观察/昆虫细胞系Key words
Daphnis nerii/primary culture/morphological observation/insect cell line分类
生物科学引用本文复制引用
李江怀,占智高,王金昌,郑国华,关丽梅,况文东,陈俊晖,杨健,靳亮..夹竹桃天蛾胚胎细胞的原代培养[J].江西科学,2023,41(6):1071-1076,6.基金项目
江西省科学院包干制重点研发计划项目(2021YSBG22011) （2021YSBG22011）
江西省科学院基础研究一般项目(2022YJC2008) （2022YJC2008）
国家自然基金地区科学基金项目(32260705) （32260705）
江西省重点研发计划项目(20212BBG73046). （20212BBG73046）
