检验医学与临床2023，Vol.20Issue(24)：3599-3602,3606,5.DOI:10.3969/j.issn.1672-9455.2023.24.004
ST2、NK细胞和B细胞在T2DM患者中的水平及相关性分析
Levels of sST2,NK cells and B cells in patients with T2DM and their correlation analysis
摘要
Abstract
Objective To explore the levels of soluble growth stimulating gene 2 protein(sST2),natural killer(NK)cells and B cells in the patients with type 2 diabetes mellitus(T2DM)and their correlation.Meth-ods Fifty inpatients with T2DM in the Affiliated Jiangning Hospital of Nanjing Medical University from Oc-tober to December 2022 were selected as the T2DM group,and 30 healthy subjects undergoing physical exami-nation in this hospital during the same period were selected as the control group.The flow cytometry was used to detect the proportions of NK cells(CD45'CD3-CD19-CD56+)and B cells(CD45+CD3-CD56-CD19+);the serum IL-6,TNF-α and IFN-γ levels were detected by the flow cytometry and immunofluorescence tech-nique;the immunofluorescence dry quantitative detection was used to detect the serum sST2 levels of all study subjects;the correlation between serum sST2 expression level with NK cells and B cells in T2DM patients,as well as the correlation between B cells and NK cells in T2 DM patients.Results The serum sST2 level in the T2DM group was higher than that in the control group,the proportions of B cells and NK cells were signifi-cantly lower than those in the control group,and the inflammatory cytokines IL-6,TNF-α and IFN-γ levels were higher than those in the control group,the differences were statistically significant(P＜0.05).The ser-um sST2 level in T2DM patients was negatively correlated with the proportions of B cells(r=-0.40,P＜0.01)and NK cells(r=-0.29,P＜0.05);the proportion of B cells in T2DM patients was positively correla-ted with the proportion of NK cells(r=0.34,P＜0.05).Conclusion The serum sST2 level,NK and B cells in T2DM patients have the correlation.关键词
可溶性生长刺激表达基因2蛋白/自然杀伤细胞/B细胞/2型糖尿病Key words
soluble growth stimulating gene 2 protein/natural killer cell/B cell/type 2 diabetes mel-litus分类
临床医学引用本文复制引用
陈莉,司徒秋晨,韦静,吕宏祥..ST2、NK细胞和B细胞在T2DM患者中的水平及相关性分析[J].检验医学与临床,2023,20(24):3599-3602,3606,5.基金项目
江苏省南京市卫生科技发展专项资金项目(YKK21228) （YKK21228）
江苏大学临床医学科技发展基金项目(JLY2021154). （JLY2021154）
