口腔颌面外科杂志2023，Vol.33Issue(6)：355-362,8.DOI:10.12439/kqhm.1005-4979.2023.06.001
Lmo7对MC3T3-E1细胞增殖、迁移及成骨分化影响的实验研究
Effect of Lmo7 on proliferation,migration and osteogenic differentiation of MC3T3-E1 cells in vitro
摘要
Abstract
Objective:To investigate the effects of Lmo7 gene in the process of proliferation,migration and osteogenic differentiation of MC3T3-E1 cells.Methods:The preosteoblasts MC3T3-E1 cells were cultured in vitro with osteogenic induction.The relative expression of Lmo7 was detected by real-time quantitative polymerase chain reaction(RT-qPCR)and Western blotting at 0,3,7 and 14 d respectively.At the same time,MC3T3-E1 cells were transfected with pLV-shLmo7 and pLV-shControl to construct the stably interfering cell line.Cell proliferation and cell migration were detected by CCK-8 assay,EdU assay,wound healing assay and Transwell migration assay.While alkaline phosphatase(ALP)staining was used to detect the expression activity of ALP,and RT-qPCR was used to detect the expression of osteogenesis-related genes such as Runt-related transcription factor 2(Runx2),Osterix,alkaline phosphatase(ALP)and osteocalcin(OCN).Results:Lmo7 gene expression level was up-regulated during the osteogenic differentiation of MC3T3-E1 cells in vitro.The stable MC3T3-E1 cell line with Lmo7 interference was successfully constructed,and its proliferation activity was significantly improved,while its migration ability was inhibited.After osteogenic induction,compared with the control group,the ALP staining of the Lmo7 interfering cells was lighter,and the expression levels of osteogenesis-related genes such as Runx2,Osterix,ALP,and OCN were significantly down-regulated(P<0.05).Conclusion:Lmo7 interference promoted the cell proliferation but inhibited the cell migration.Furthermore,the osteogenic differentiation ability of cells was suppressed.关键词
Lmo7/MC3T3-E1细胞/RNA干扰Key words
Lmo7/MC3T3-E1 cells/RNA interference分类
医药卫生引用本文复制引用
毛佳奕,王佐林..Lmo7对MC3T3-E1细胞增殖、迁移及成骨分化影响的实验研究[J].口腔颌面外科杂志,2023,33(6):355-362,8.基金项目
国家自然科学基金(81670962) （81670962）
国家重点研发计划(2018YFE0202200) （2018YFE0202200）
中央高校基本业务费专项资金(22120180196) （22120180196）
