口腔颌面外科杂志2023，Vol.33Issue(6)：363-369,7.DOI:10.12439/kqhm.1005-4979.2023.06.002
MMP-9抑制剂对人口腔鳞状细胞癌细胞系CAL27生物学行为影响机制的研究
Study on the mechanism of MMP-9 inhibitor on biological behavior of human oral squamous cell carcinoma cell line CAL27
摘要
Abstract
Objective:To explore the potential of BB-94,a matrix metalloproteinase-9(MMP-9)inhibitor,in alleviating malignant biological behavior of human oral squamous cell carcinoma(OSCC)cell line CAL27 and its possible mechanism.Methods:CAL27 cells in logarithmic growth phase were cultured in BB-94 culture medium with different concentrations(0,0.125,0.25,0.5,1.0 µg/mL)for 48 hours;the activity of MMP-9 in cell culture supernatant was analyzed by gelatin zymogram;the cell survival rate was detected by cell counting kit-8(CCK-8)method;the apoptosis,migration,and invasion of CAL27 cells were detected by flow cytometry,wound healing test and Transwell test;real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the mRNA expression levels of phosphatidylinositol 3-kinase(PI3K)and protein kinase B(Akt);the expressions of PI3K,phosphorylated protein kinase B(p-Akt),MMP-9,vimentin(Vim),N-cadherin(N-cad)and E-cadherin(E-cad)proteins were detected by Western blotting.Results:The relative activity of MMP-9,the survival rate of CAL27 cells,the wound healing rate,the number of cell invasion,the relative expression level of PI3K and Akt mRNA and the relative expression level of PI3K,p-Akt,Vim,N-cad and MMP-9 protein decreased with the increase in the concentration of BB-94(P<0.05).The apoptosis rate of CAL27 cells and the relative expression level of E-cad protein increased as the concentration of BB-94 went up(P<0.05).Conclusion:These results indicate that MMP-9 inhibitor could inhibit the malignant biological behavior of human OSCC cell line CAL27,and its mechanism may be related to preventing the activation of PI3K/Akt signaling pathway and the progress of epithelial mesenchymal transition(EMT).关键词
基质金属蛋白酶-9抑制剂/人口腔鳞状细胞癌/恶性生物学行为/磷脂酰肌醇3-激酶/蛋白激酶BKey words
matrix metalloproteinase-9 inhibitor/human oral squamous cell carcinoma/malignant biological behavior/phosphatidylinositol 3-kinase/protein kinase B分类
医药卫生引用本文复制引用
王静璇,刘红悦,庄志征,胡燕,杨英顺..MMP-9抑制剂对人口腔鳞状细胞癌细胞系CAL27生物学行为影响机制的研究[J].口腔颌面外科杂志,2023,33(6):363-369,7.基金项目
2021年度河北省医学科学研究课题计划(20210409) （20210409）
