口腔颌面外科杂志2023，Vol.33Issue(6)：370-378,9.DOI:10.12439/kqhm.1005-4979.2023.06.003
4-辛基衣康酸抑制脂多糖诱导的小鼠颅骨炎性骨吸收的效果研究
The effect of 4-octyl itaconate on lipopolysaccharide-induced murine calvarial osteolysis
摘要
Abstract
Objective:To investigate the effect of 4-octyl itaconate(4-OI)on lipopolysaccharide(LPS)-induced calvarial osteolysis in mice.Methods:CCK-8 assay was used to evaluate the effect of different concentrations of 4-OI on the cell viability of RAW 264.7 macrophages and murine primary bone marrow-derived macrophages(BMMs).Real-time quantitative polymerase chain reaction(RT-qPCR),immunofluorescence staining,and intracellular reactive oxygen species(ROS)detection kit(DCFH-DA)were used to explore the effect of 4-OI at different concentrations(12.5 µmmol/L and 50 µmmol/L)on the inflammatory response of macrophages.Tartrate-resistant acid phosphatase(TRAP)staining was used to explore the effect of 4-OI on osteoclast differentiation.The murine LPS-induced mouse calvarial osteolysis model was established,and the therapeutic effect of 4-OI on inflammatory osteolysis was evaluated by micro-CT and histological staining.Results:The results of CCK-8 showed that 4-OI below a concentration of 50 µmmol/L had no obvious cytotoxicity to RAW 264.7 macrophages and murine BMMs.RT-qPCR and immunofluorescence results showed that 4-OI was concentration-dependent,and inhibited the expression of pro-inflammatory cytokines.TRAP staining showed that 4-OI effectively inhibited the osteoclast differentiation induced by receptor activator of nuclear factor κB ligand(RANKL).Micro-CT and histological staining demonstrated that 4-OI effectively alleviated the inflammatory response and bone resorption around the osteolysis site.Conclusion:4-OI can effectively inhibit inflammatory osteolysis,and it has great potential application in the treatment of osteolysis related diseases in oral and maxillofacial region.关键词
4-辛基衣康酸/炎性骨溶解/巨噬细胞/脂多糖Key words
4-octyl itaconate/inflammatory osteolysis/macrophage/lipopolysaccharide分类
医药卫生引用本文复制引用
温从鹏,童无忧,陈旭卓,赖林锋..4-辛基衣康酸抑制脂多糖诱导的小鼠颅骨炎性骨吸收的效果研究[J].口腔颌面外科杂志,2023,33(6):370-378,9.基金项目
温州市科研项目(Y20220380) （Y20220380）
