兰州大学学报（医学版）2023，Vol.49Issue(10)：16-25,10.DOI:10.13885/j.issn.1000-2812.2023.10.003
SOCS3受miR-183-5p调控影响乳腺癌细胞的增殖、迁移、侵袭和凋亡
MiR-183-5p promotes proliferation,metastasis and inhibits apoptosis of breast cancer cells by targeting SOCS3
褚旭 1赵浩 1向玲 1邹燕1
作者信息
- 1. 自贡市第四人民医院 甲状腺乳腺外科,四川 自贡 643000
- 折叠
摘要
Abstract
Objective To explore the molecular mechanism of SOCS3 in regulating the progression of breast cancer.Methods Bioinformatics methods were used to analyse the data of breast cancer patients in the TCGA database;breast cancer cell lines were cultured in vitro,the mRNA expression levels of SOCS3 and miR-183-5p detected by qRT-PCR and the protein expression level of SOCS3 detected by protein blotting;MDA-MB-231 and T47D cells were treated with knockdown or overexpression of SOCS3 and miR-183-5p,dual luciferase reporter assay used to test the target-binding relationship of SOCS3 and miR-183-5p;CCK-8 and clone formation assay was used to detect the proliferative ability of MDA-MB-231 and T47D cells;transwell assay used to detect the migratory and invasive ability of MDA-MB-231 and T47D cells;flow cytometry used to detect the apoptotic rate of MDA-MB-231 and T47D cells.Results SOCS3 was lowly expressed in breast cancer tissues and cell lines,whereas miR-183-5p was highly expressed.The cell function experiment proved that SOCS3 inhibited the proliferation,migration and invasion and promoted the apoptosis of breast cancer cells.Bioinformatics analysis of the starBase and Targetscan databases revealed that miR-183-5p has binding sites with SOCS3.Meanwhile,dual luciferase reporting experiments demonstrated a targeted binding relation-ship between miR-183-5p and SOCS3.MiR-183-5p downregulates SOCS3 expression in a targeted manner.Cell function assay experiments showed that down-regulation of miR-183-5p with simultaneous silencing of SOCS3 resulted in significantly higher proliferation,migration and invasion abilities and lower apoptosis rates compared to down-regulation of miR-183-5p alone,whereas cells overexpressing miR-183-5p and silencing of SOCS3 showed further higher proliferation,migration and invasion abilities and lower apoptosis rates com-pared to simultaneous silencing of miR-183-5p and SOCS3.Conclusion SOCS3 inhibits proliferation,migra-tion,invasion and promotes apoptosis of breast cancer cells and is regulated by miR-183-5p targeting.关键词
乳腺癌/细胞因子信号传送阻抑物/miR-183-5p/增殖/迁移和侵袭/凋亡Key words
breast cancer/suppressor of cytokine signaling 3/miR-183-5p/proliferation/migration and inva-sion/apoptosis分类
医药卫生引用本文复制引用
褚旭,赵浩,向玲,邹燕..SOCS3受miR-183-5p调控影响乳腺癌细胞的增殖、迁移、侵袭和凋亡[J].兰州大学学报（医学版）,2023,49(10):16-25,10.
