兰州大学学报（医学版）2023，Vol.49Issue(10)：32-39,8.DOI:10.13885/j.issn.1000-2812.2023.10.005
聚乙二醇修饰的可控温磁性纳米颗粒制备与表征研究
Synthesis and characterization of polyethylene glycol-coated self-regulated magnetic nanoparticles
摘要
Abstract
Objective To investigate magnetic nanoparticles(MNPs)as important carriers for cancer diagnosis and therapy because they can generate heat under an alternating magnetic field to kill tumor cells.Regulating the Curie temperature of MNPs can enhance their safety for magnetic hyperthermia cancer treatment.Methods Self-regulated Zn0.54Co0.46Cr0.6Fe1.4O4 were synthesized via the hydrothermal method,and then modi-fied by polyethylene glycol(PEG)with a molecular weight of 20 000.Transmission electron microscope(TEM),X-Ray diffractometer(XRD)and vibrating sample magnetometer(VSM)were utilized to characterize the size,morphology,crystal structure and magnetic properties of MNPs.The cytotoxicity of pristine and PEG-modified MNPs toward normal human extrahepatic bile duct epithelial cells(HEBEpiC)was evaluated by the cell counting kit-8(CCK-8)assay.Results TEM results revealed a significant aggregation in un-modified Zn0.54Co0.46Cr0.6Fe1.4O4 nanoparticles while PEG-modified Zn0.54Co0.46Cr0.6Fe1.4O4@PEG20000 exhibited relatively uniform quasi-spherical morphology with improved dispersibility.XRD results revealed that MNPs possessed the characteristic diffraction peaks of the cubic spinel structure of CoFe2O4 and contained Zn2+,Co2+,Cr3+,and Fe3+.VSM results demonstrated good superparamagnetism of MNPs.The CCK-8 assay results demonstrated that neither form of MNPs exhibited significant toxicity toward HEBEpiC cells across the tested concentration range.Compared to the MNPs group,the PEG-modified MNPs showed slightly higher cell viability of HEBEpiC,indicating the PEG helped further reduce the cytotoxicity of MNPs toward HEBEpiC cells.Conclusion This study indicates that PEG coating could significantly improve the dispersibility,magnetothermal properties and cytotoxicity of Zn0.54Co0.46Cr0.6Fe1.4O4,laying the foundation for their potential biomedical applications in drug delivery,diagnosis and therapy.关键词
可控温磁性纳米颗粒/聚乙二醇/磁感应热疗/纳米材料/纳米医学Key words
self-regulated magnetic nanoparticles/polyethylene glycol/magnetic hyperthermia/nanomateri-als/nanomedicine分类
数理科学引用本文复制引用
张先卓,林延延,叶为春,孟文勃,逯娅雯,谭琳,马泽龙,姜宁祖,赵进玉,田亮,张保平,王艺霖..聚乙二醇修饰的可控温磁性纳米颗粒制备与表征研究[J].兰州大学学报（医学版）,2023,49(10):32-39,8.基金项目
甘肃省卫生健康行业科研资助项目(GSWSQN2021-001) （GSWSQN2021-001）
甘肃省卫生健康行业科研计划资助项目(GSWSKY2020-11) （GSWSKY2020-11）
兰州大学医学教育创新发展资助项目(lzuyxcx-2022-157) （lzuyxcx-2022-157）
