兰州大学学报（医学版）2023，Vol.49Issue(10)：60-72,13.DOI:10.13885/j.issn.1000-2812.2023.10.009
基于氧化应激因子构建结肠癌的预后风险标志物及其临床意义
Construction of prognostic risk markers of colon cancer based on oxidative stress factors and its clinical significance
摘要
Abstract
Objective To investing the feature genes related to oxidative stress in colon cancer and screen prognostic markers of colon cancer.Methods Oxidative stress related genes were combined with transcrip-tomic data of colon cancer to screen prognostic markers,followed by construction of a prognostic model using Cox regression analysis.The validity of the model was analyzed by survival analysis and receiver operating characteristic curves.High-and low-risk groups were distinguished according to the median risk-score.Cox analysis was used to verify the independence of risk-score,followed by the construction of nomograms.Finally,the prognostic differences,immune microenvironment differences,and the degree of response to immunotherapy between the high-and low-risk groups were analyzed.Results Nine oxidative stress related genes(CPT2,FARS2,RYR2,MSRA,PPARGC1a,LEP,AGT,EPO,STK25)were screened and used to con-struct a prognostic model.The results of survival analysis and receiver operating characteristic curve showed that the model could predict risk of colon cancer well(P<0.05,area under curve>0.6).Risk-score could be used as an independent prognostic factor(P<0.001).Additionally,the frequency of TP53 mutations was high-er in the high-risk group(P<0.000 1).Meanwhile,the immunopheno scores was higher in the low-risk group than in the high-risk group(P<0.05).And tumor immune dysfunction and exclusion scores in the low-risk group was lower than that in the high-risk group(P<0.05).Finally,The overall survival of colon cancer patients predicted by nomograms at 1 and 3 years is very close to the actual overall survival.Conclusion This study identified oxidative stress related prognostic markers for colon cancer,which may be new potential therapeutic targets for the treatment of colon cancer patients.关键词
氧化应激因子/结肠癌/生物信息学分析/预后标志物/免疫治疗Key words
oxidative stress factor/colon cancer/bioinformatics analysis/prognostic markers/immunotherapy分类
医药卫生引用本文复制引用
李轶辉,许玉春,彭浩,郭蓉,龚维..基于氧化应激因子构建结肠癌的预后风险标志物及其临床意义[J].兰州大学学报（医学版）,2023,49(10):60-72,13.基金项目
湖南省教育厅课题(20C1116) （20C1116）
