临床口腔医学杂志2023，Vol.39Issue(12)：712-717,6.DOI:10.3969/j.issn.1003-1634.2023.12.003
基于NF-κB信号通路探究SIRT7基因对口腔癌细胞株自噬蛋白及巨噬细胞极化的作用机制
Study on the mechanism of SIRT7 gene on autophagy protein and macrophage polarization of oral cancer cell line based on NF-κB signal pathway
摘要
Abstract
Objective:To explore the mechanism of SIRT7 gene on autophagy protein and macrophage polarization in oral squamous cell carcinoma cells based on the NF-κB signaling pathway.Methods:Human oral squamous cell carcinoma cell line SCC25 cells were divided into SCC25 group(SCC25 cells without intervention),negative control group(SCC25 cells transfected with empty vector pLKO.1-vector-GFP),SIRT7 shRNA group(SCC25 cells transfected with SIRT7 shRNA),SIRT7 group(SCC25 cells transfected SIRT7 lentivirus),combination group A(SCC25 cells +50 μmol/L NF-κB inhibitor PDTC+ SIRT7 shRNA)and combination group B(SCC25 cells +50 μmol/L NF-κB inhibitor PDTC+SIRT7 lentivirus),the relative expression of SIRT7 was detected by qRT-PCR.The number of invasion was detected by Transwell method.The scratch heal-ing rate was detected by scratch test.Immunofluorescence detection of P62,LC3-Ⅱ,the levels of M1 and M2 marker proteins,NF-κB and NF-κB p65 proteins were detected by Western blot.Results:There was no significant difference in SCC25 cells invasion number,scratch healing rate,P62,LC3-Ⅱ,CD163,CD11C,NF-κB and NF-κB p65 expression between SCC25 group and negative control group(P>0.05).The number of cell invasion,scratch healing rate,CD163,NF-κB and NF-κB p65 were increased and CD11C was decreased in SIRT7 shRNA group(P<0.05).The invasion number,scratch healing rate,CD163,NF-κB and NF-κB p65 of SCC25 cells in SIRT7 group were decreased,while CD11C was increased(P<0.05).Compared with combination group A,the invasion number,scratch healing rate,CD163,NF-κB and NF-κB p65 of SCC25 cells in com-bination group B were decreased,and CD11C was increased(P<0.05).Conclusion:Up-regulation of SIRT7 can inhibit the invasion and migration of oral squamous cell cancer cells,reduce the activities of autophagy protein P62 and LC3-Ⅱ,and pro-mote the polarization of macrophage M2 to M1.The mechanism may be related to the regulation of NF-κB by SIRT7.关键词
口腔癌/SIRT7/自噬蛋白/巨噬细胞极化/核转录因子NF-κBKey words
Oral cancer/SIRT7/Autophagy protein/Macrophage polarization/Nuclear transcription factor NF-κB分类
医药卫生引用本文复制引用
顾超,张红梅,仝越越,孙红玲,何晓娉..基于NF-κB信号通路探究SIRT7基因对口腔癌细胞株自噬蛋白及巨噬细胞极化的作用机制[J].临床口腔医学杂志,2023,39(12):712-717,6.基金项目
国家自然科学基金项目(编号:81502340) （编号:81502340）
