临床与实验病理学杂志2023，Vol.39Issue(11)：1305-1310,6.DOI:10.13315/j.cnki.cjcep.2023.11.005
ALK(1A4)在ALK融合非小细胞肺癌诊断中的应用
Application of ALK antibody clone 1A4 in the diagnosis of non-small cell lung cancer with ALK fusion
摘要
Abstract
Purpose The aim of this study is to explore the application value of ALK(1A4)in ALK fused non-small cell lung cancer(NSCLC).Methods A total of 1 035 NSCLC specimens were collected.The expression of ALK(1A4)and ALK(D5F3)antibodies was detected by immunohistochemical(IHC)staining,and their consistency was analyzed.FISH and next-generation sequencing(NGS)were used to detect ALK positive,and the sensitivity and specificity of ALK in the two groups were evaluated.Results ALK fused NSCLC patients accounted for about 5.4％(56/1 035).The positive rate of ALK(1A4)was 7.2％(75/1 035),and that of ALK(D5F3)was 5.7％(59/1 035).The consistency between them was high,with a Kappa value of 0.874.The consistency of ALK(1A4)and ALK(D5 F3)antibodies with FISH was high,with Kappa values of 0.845 and 0.954,respectively.The consisten-cy of ALK(1A4)and ALK(D5F3)with NGS was also high,with Kappa values of 0.836 and 0.988,respectively.According to the FISH results,the sensitivities of ALK(1A4)and ALK(D5F3)antibodies were 100％and 98.2％,and the specifici-ties were 98.1％and 99.6％,respectively.Conclusion ALK(1A4)antibody has high sensitivity and slightly low specificity,and can be used for clinical screening of ALK fused NSCLC.关键词
肺肿瘤/非小细胞肺癌/ALK/ALK(1A4)抗体/ALK(D5F3)抗体/免疫组织化学Key words
lung neoplasm/non-small cell lung cancer/ALK/ALK(1A4)antibody/ALK(D5 F3)antibody/immunohisto-chemistry分类
临床医学引用本文复制引用
王悦,金燕,郑强,薛倩倩,蒋文华,蔡旭,李媛..ALK(1A4)在ALK融合非小细胞肺癌诊断中的应用[J].临床与实验病理学杂志,2023,39(11):1305-1310,6.基金项目
国家自然科学基金面上项目(81972171) （81972171）
