辽宁农业科学Issue(6)：6-11,6.DOI:10.3969/j.issn.1002-1728.2023.06.002
野蔷薇花瓣转录组分析
Transcriptome Analysis and Differential Gene Screening at Different Opening Stages of Rose multiflora Thunb.Petals
摘要
Abstract
This experiment aimed to investigate the gene expression related to petal expansion during the open-ing of Rose multiflora petals.To achieve this,RNA-seq analysis was performed on petals at various stages of o-pening.The results of this study provide basis for further exploration of the transcriptional regulation of Rose multiflora petal development.The results indicated that:The transcriptome analysis resulted in the assembly of 52 865 Unigenes.These were functionally annotated by comparing them to the NR database,and were found to match with various species.The top three species that matched were Fragaria vesca subsp.vesca(36.17%),Oryza sativa Japonica(13.65%),and Gossypium hirsutum(4.04%).A total of 642 902 and 1 899 differential genes were identified in the three stages of petal opening(FB_CPvsFB_GP,FB_YPvsFB_CP,OF_WPvsFB_YP).By analyzing the expression of genes related to petal expansion at different flowering stages.These genes may be influenced by EXP,XTH gene family,XYL,and CES genes related to cell wall synthesis,modification,and hydrolysis.关键词
野蔷薇/转录组/花朵开放/花瓣扩展Key words
Rose multiflora Thunb./Transcriptome/Flowers open/Petal expansion分类
农业科技引用本文复制引用
熊燕,陈菲,李黎,付俊生,韩辉,曲彦婷..野蔷薇花瓣转录组分析[J].辽宁农业科学,2023,(6):6-11,6.基金项目
黑龙江省科学院科学研究基金(KY2021ZR05) （KY2021ZR05）
黑龙江省省属科研院所科研业务费项目(CZKYF2021-2-C008) （CZKYF2021-2-C008）
黑龙江省科学院双提雁阵特别计划(STY2022ZR01) （STY2022ZR01）
黑龙江省科学院人才队伍建设平台项目(RC2022ZR01) （RC2022ZR01）
