林业科学2023，Vol.59Issue(12)：125-136,12.DOI:10.11707/j.1001-7488.LYKX20230447
植物促生微生物对枫香叶色素组成和根际微生物群落的影响
Effects of Plant Growth-Promoting Microorganisms on Rhizosphere Microbial Community and the Leaf Pigment Composition of Liquidambar formosana
摘要
Abstract
[Objective]In this study,plant growth-promoting microorganisms(PGPM)were used to inoculate into the rhizosphere of Liquidambar formosana to investigate effects of PGPM on soil physicochemical properties and the leaf color of L.formosana,as well as changes in the composition and diversity characteristics of the rhizosphere microbial community,in order to study the effect of PGPM on plants and soil microorganisms.[Method]A field inoculation experiment was conducted on 7-year-old L.formosana seedlings with consistent growth in Jurong Forest Farm of Jurong City with phosphate solubilizing bacteria Serratia marcescens subsp.marcescens(NJ2D)and Funneliformis mosseae(BJ04),and the effects on soil physical and chemical properties,leaf color parameters in autumn and soil microbial community were analyzed.[Result]1)The total nitrogen,total phosphorus,soil organic matter,available phosphorus content,soil moisture content(%),and soil pH of the rhizosphere soil of L.formosana in the treatment group were significantly increased compared to the control group(P<0.05).2)The content of chlorophyll a,chlorophyll b,total chlorophyll,and carotenoids in leaves of L.formosana inoculated once with BJ04 significantly increased(P<0.05),while NJ2D+BJ04 inoculation significantly increased the anthocyanin level in the leaves(P<0.05).3)There were significant differences in the rhizosphere soil microbial community diversity between control group(CK),NJ2D,BJ04 and NJ2D+BJ04 treatments(P<0.05).The number of OTUs(operating taxonomic units)of rhizosphere bacteria in L.formosana trees inoculated with NJ2D,BJ04,and NJ2D+BJ04 was significantly increased compared to the control group(P<0.05),while the number of OTUs of arbuscular mycorrhizal fungi(AMF)was significantly reduced compared to the control group(P<0.05).4)The high-throughput sequencing results of rhizosphere microorganisms showed that Actinobacteria,Proteobacteria,Chloroflexi,Acidobacteria and Gemmatimonadetes dominated the bacterial communities on the rhizosphere of L.formosana.Within the AMF community,Glomus,Rhizophagous and Dominika were dominant genera.However,after inoculation,the species abundances of Acidobacteria,Chloroflexi and Planctomycetes were significantly reduced within the rhizosphere of L.formosana(P<0.05),whereas Proteobacteria and Actinobacteria were significantly increased(P<0.05).[Conclusion]Inoculation with phosphorus solubilizing bacteria,arbuscular mycorrhizal fungi,and composite microbial agents NJ2D+BJ04 can all influence the soil microenvironment and have an impact on the autumn colour of L.formosana leaves.关键词
根际微生物群/土壤理化性质/叶色素/微生物互作/枫香Key words
rhizosphere microbiome/soil physicochemical properties/leaf pigment/biological interaction/Liquidambar formosana分类
农业科技引用本文复制引用
吴祝华,宋娟,朱树林,赵邢,杨学祥,任嘉红,陈凤毛..植物促生微生物对枫香叶色素组成和根际微生物群落的影响[J].林业科学,2023,59(12):125-136,12.基金项目
国家自然科学基金项目(31100471) （31100471）
江苏省高等学校优先学术计划(PAPD) （PAPD）
江苏省林业科技创新与推广项目(LYKJ[2017]20). （LYKJ[2017]20）
