中南医学科学杂志2023，Vol.51Issue(6)：808-812,5.DOI:10.15972/j.cnki.43-1509/r.2023.06.003
褪黑素抑制人血管平滑肌细胞的巨噬样表型转化
Melatonin inhibits the macrophage-like phenotype transformation of human vascular smooth muscle cells
摘要
Abstract
Aim To establish a macrophage-like phenotypic transformation model of human vascular smooth muscle cells(VSMC)induced by water-soluble cholesterol,and to explore the role of melatonin in this process.Meth-ods VSMC is divided into a control group,a cholesterol group(water-soluble cholesterol),and a melatonin group(water-soluble cholesterol+melatonin).Establishment of a macrophage like phenotype transformation model in VSMC induced by water-soluble cholesterol.The expression of macrophage-like phenotypic related genes in VSMC was detected by quantitative PCR.Oil red O staining experiment was conducted to observe the effect of melatonin on the lipid accumulation of VSMC in-duced by water-soluble cholesterol.Edu staining was used to observe the effect of melatonin on the proliferation of VSMC induced by water-soluble cholesterol.The effect of melatonin on VSMC migration induced by water soluble cholesterol was evaluated by cell scratch assay.Results The mRNA expression of smooth musclecontraction phenotype related mark-ers,α-actin and α-tropomyosin,were significantly decreased after 10 mg/L water-soluble cholesterol treatment,while the mRNA expression macrophage related markers,CD68 and MAC2,were significantly increased after treated by 10 mg/L wa-ter-soluble cholesterol,suggesting the transformation of VSMC to a macrophage-like phenotype.In addition,0.5 μmol/L melatonin inhibited lipid accumulation and proliferation of VSMC induced by water-soluble cholesterol,but had no effect on VSMC migration.Conclusion Melatonin inhibits the macrophage-like phenotype transformation of VSMC.关键词
动脉粥样硬化/血管平滑肌细胞/巨噬样表型转化/褪黑素Key words
As/vascular smooth muscle cells/macrophage-like phenotype transformation/melatonin分类
医药卫生引用本文复制引用
李双,谭翠钰,侯连杰,赵国军..褪黑素抑制人血管平滑肌细胞的巨噬样表型转化[J].中南医学科学杂志,2023,51(6):808-812,5.基金项目
国家自然科学基金面上项目(81870337,82300517) （81870337,82300517）
广州医科大学2022年度学生创新能力提升计划项目(2022-66) （2022-66）
