中南医学科学杂志2023，Vol.51Issue(6)：813-817,5.DOI:10.15972/j.cnki.43-1509/r.2023.06.004
巨噬细胞ADAM8诱导血管平滑肌细胞去分化
Macrophage ADAM8 induced dedifferentiation of vascular smooth muscle cells
摘要
Abstract
Aim To study the effect of macrophage recombinant A disintegrin and metalloprotease 8(ADAM8)overexpression on dedifferentiation of vascular smooth muscle cells.Methods The differentially expressed genes of human aortic dissection and mouse abdominal aortic aneurysm were screened by bioinformatics analysis.ADAM8 was overexpressed in macrophages,and macrophage medium was collected to treat vascular smooth muscle cells.The expres-sion of ADAM8 was detected by qPCR and Western blotting.The migration ability of vascular smooth muscle cells was detected by cell scratch assay.The proliferative ability of vascular smooth muscle cells was evaluated by Edu staining.The effect of macrophage culture medium over expressing ADAM8 on the expression of differentiation marker genes in vas-cular smooth muscle cells was detected by qPCR.Results The ADAM8 gene was significantly upregulated during the process of human thoracic aortic dissection and mouse abdominal aortic aneurysm lesions(P<0.05),and the expression of ADAM8 was also significantly increased during the inflammatory response of macrophages induced by lipopolysaccharide(LPS)(P<0.05).Overexpression of ADAM8 by macrophages induces cellular inflammatory response and matrix metal-loproteinases(MMP)expression.In contrast,overexpressing ADAM8 in macrophage culture medium promotes the mi-gration and proliferation ability of vascular smooth muscle cells,but significantly inhibits the expression of vascular smooth muscle cell differentiation marker genes α-action and α-tropomyosin(P<0.05).Conclusion Macrophage ADAM8 induces dedifferentiation of vascular smooth muscle cells by increasing the expression of inflammatory cytokines and MMP.关键词
巨噬细胞/ADAM8/血管平滑肌细胞/增殖/迁移Key words
macrophage/ADAM8/vascular smooth muscle cells/proliferation/migration分类
医药卫生引用本文复制引用
邓洁锋,米娅丽,侯连杰,赵国军..巨噬细胞ADAM8诱导血管平滑肌细胞去分化[J].中南医学科学杂志,2023,51(6):813-817,5.基金项目
国家自然科学基金面上项目(81870337) （81870337）
广州医科大学学生创新能力提升计划项目(2022-66) （2022-66）
