过表达F-box基因BoFBX117对甘蓝低温耐受性的影响OACSTPCD

F-box gene plays an important role in plant abiotic stress response.In order to study the function of F-box gene BoFBX117(GenBank No.XM_013731217.1)in cabbage(Brassica oleracea var.capitata)response to low-temperature stress,in this study,the BoFBX117 over-expression vector was constructed,and the over-expression of BoFBX117 cabbage(OE-BoFBX117)plants were obtained by Agrobacterium tumefaciens mediated method.After 24 h of low-temperature stress at 4,0,-2 and-4℃,the phenotype of the over-expression of BoFBX117 cabbage showed that the degree of leaf yellowing wilting was less than that of non-transgenic cabbage(WT).After 7 d of recovery,it was found that the over-expression of BoFBX117 cabbage had stronger ability to return to normal growth.The results of qPCR showed that the expression of BoFBX117 in transgenic plants was significantly increased under low-temperature stress(P＜0.01),which was 1.87～4.80 times that of WT plants.Under low-temperature stress at 0℃,the activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)of transgenic plants were the highest,which were 80%,16%and 99%higher than those of WT plants respectively.Under low-temperature stress,the content of malondialdehyde(MDA)continuously accumulated.At-4℃,the MDA content of transgenic plants was 0.62 μmol/g FW,53.38%lower than WT.Under normal conditions and low-temperature stress,the proline and soluble sugar contents of transgenic plants were significantly higher than those of WT plants(P＜0.05).The results showed that BoFBX117 might play a positive role in the response to low-temperature stress,and over-expression of BoFBX117 in cabbage could improve the low-temperature tolerance of cabbage.This study provides support for the breeding of low-temperature tolerance varieties of cabbage.