农业生物技术学报2024，Vol.32Issue(1)：189-199,11.DOI:10.3969/j.issn.1674-7968.2024.01.017
PEX10基因缺失对兔须癣毛癣菌生长及致病性的抑制作用
Inhibitory Effect of PEX10 Gene Deletion on the Growth and Pathogenicity of Trichophyton mentagrophytes in Oryctolagus cuniculus
摘要
Abstract
Trichophyton mentagrophytes,also known as T.gypsioides,is severely hazardousness to rabbit(Oryctolagus cuniculus).Peroxisomal biogenesis factor 10(PEX10)gene is closely related to the drug susceptibility of plant pathogenic fungi.However,the mechanism of whether PEX10 gene affects the growth and pathogenicity of animal pathogenic fungi such as T.mentagrophytes is still unclear.This study first constructed a PEX10 gene knockout vector and obtained a PEX10 gene knockout strain of T.mentagrophytes through Agrobacterium tumefaciens-mediated transformation(AtMT)and transformant screening.Phenotypic analysis such as growth characteristics showed that the growth rate of PEX10 gene knockout strains was significantly slowed down(P＜0.01),and their sensitivity to berberine hydrochloride was significantly increased(P＜0.001).The virulence and pathogenicity of the PEX10 knockout strain to rabbit skin was reduced.The results of this study provide reference for the study of PEX10 gene function and the prevention and treatment of T.mentagrophytes of rabbits.关键词
须癣毛癣菌/过氧化物酶体生物合成因子10(PEX10)/过氧化物酶体/耐药性Key words
Trichophyton mentagrophytes/Peroxisomal biogenesis factor 10(PEX10)/Perxisome/Drug resistance分类
农业科技引用本文复制引用
潘瑶,王教瑜,鲍国连,肖琛闻,刘燕,张蓬军,应淑敏,黄叶娥,季权安,韦强,王静..PEX10基因缺失对兔须癣毛癣菌生长及致病性的抑制作用[J].农业生物技术学报,2024,32(1):189-199,11.基金项目
国家兔产业技术体系岗位专家项目(CARS-43-C-2) （CARS-43-C-2）
浙江省重点研发计划项目(2021C02068-7) （2021C02068-7）
