农业生物技术学报2024，Vol.32Issue(1)：209-219,11.DOI:10.3969/j.issn.1674-7968.2024.01.019
纳米抗体结构特征与制备研究进展
Research Progress on Structural Characteristics and Preparation of Nanobodies
摘要
Abstract
Nanobodies (Nb) are the smallest known fragments composed of only heavy chains, which can bind to antigens and have broad application prospects. In this paper, three different variable region of subtypes of antibodies in Camelidae animals were introduced, and the similarities and differences between traditional antibodies IgG and nanobodies in the types and positions of disulfide bonds, the composition of skeleton regions, and complementary determining regions were revealed. The characteristics of nanobodies, such as simple production, strong specificity, high stability, and high solubility, were discussed. The screening and expression methods of Nb were expounded, that is, phage display, yeast two-hybrid and other technologies can be used to screen and obtain specific nanobodies. Because of its simple structure, Nb can be expressed in Escherichia coli and yeast (Saccharomyces) and other microbes, thus its function can be studied better. This review provides a theoretical basis for the development and application of nanobodies in the future.关键词
纳米抗体/结构特征/制备Key words
Nanobody/Structural characteristics/Preparation分类
农业科技引用本文复制引用
孔静,郑楠,王加启,赵圣国..纳米抗体结构特征与制备研究进展[J].农业生物技术学报,2024,32(1):209-219,11.基金项目
国家自然科学基金(32272888) （32272888）
国家奶牛产业技术体系(CARS-36) （CARS-36）
