花鲈半乳糖凝集素基因家族的鉴定及在不同环境因子胁迫下表达响应OACSTPCD

To investigate the roles of Galectin genes of spotted sea bass(Lateolabrax maculatus)in responding different environmental stressing,14 Galectin genes(LGALS1,LGALS2a,LGALS2b,LGALS3a,LGALS3ba,LGALS3bb,LGALS4,LGALS8a,LGALS8b,LGALS9,LGALS17,GRPa,GRPb and GRPc)were identified in this study from its genome.The accuracy of gene annotation and structural conservation were verified by phylogenetic,copy number,collinearity and gene structure analyses.RNA-seq data were used to analyze their tissue-specific expressions and their expressional response to different environmental stressing.Tissue-specific expression profiles indicated that the Galectin genes were widely expressed in 7 tissues(liver,gill,spleen,stomach,brain,testis and ovary),and 6 genes(LGALS3ba,LGALS4,LGALS8a,LGALS9,LGALS17 and GRPa)showed obvious tissue specificity.Different members of the Galectin gene family showed different levels of expression in responding four environmental factor stresses(hypoxia,alkalinity,heat and salinity acclimation),among them LGALS3a,LGALS3ba,LGALS4,LGALS17 and GRPb showed significantly differential expression in the four environmental stresses,indicating that their extensive involvement in the response and regulation of environmental stresses.