中国海洋大学学报（自然科学版）2024，Vol.54Issue(1)：35-47,13.DOI:10.16441/j.cnki.hdxb.20220380
花鲈半乳糖凝集素基因家族的鉴定及在不同环境因子胁迫下表达响应
Identification of the Galectin Genes in Spotted Sea Bass(Lateolabrax maculatus)and Their Expressions Under Different Environmental Stressing
摘要
Abstract
To investigate the roles of Galectin genes of spotted sea bass(Lateolabrax maculatus)in responding different environmental stressing,14 Galectin genes(LGALS1,LGALS2a,LGALS2b,LGALS3a,LGALS3ba,LGALS3bb,LGALS4,LGALS8a,LGALS8b,LGALS9,LGALS17,GRPa,GRPb and GRPc)were identified in this study from its genome.The accuracy of gene annotation and structural conservation were verified by phylogenetic,copy number,collinearity and gene structure analyses.RNA-seq data were used to analyze their tissue-specific expressions and their expressional response to different environmental stressing.Tissue-specific expression profiles indicated that the Galectin genes were widely expressed in 7 tissues(liver,gill,spleen,stomach,brain,testis and ovary),and 6 genes(LGALS3ba,LGALS4,LGALS8a,LGALS9,LGALS17 and GRPa)showed obvious tissue specificity.Different members of the Galectin gene family showed different levels of expression in responding four environmental factor stresses(hypoxia,alkalinity,heat and salinity acclimation),among them LGALS3a,LGALS3ba,LGALS4,LGALS17 and GRPb showed significantly differential expression in the four environmental stresses,indicating that their extensive involvement in the response and regulation of environmental stresses.关键词
花鲈/Galectin基因家族/表达分析/胁迫响应Key words
spotted sea bass/Galectin gene family/expression analysis/stress response分类
农业科技引用本文复制引用
郑圆,温海深,李吉方,方秀,王灵钰,张冲,陶泽鑫,张永航,李昀..花鲈半乳糖凝集素基因家族的鉴定及在不同环境因子胁迫下表达响应[J].中国海洋大学学报（自然科学版）,2024,54(1):35-47,13.基金项目
国家重点研究发展计划项目(2020YFD0900204) （2020YFD0900204）
国家现代农业产业技术体系项目(CARS-47)资助 Supported by the National Key Research and Development Program of China(2020YFD0900204) （CARS-47）
the China Modern Agriculture Re-search System(CARS-47) （CARS-47）
