中国海洋大学学报（自然科学版）2024，Vol.54Issue(1)：48-57,10.DOI:10.16441/j.cnki.hdxb.20220375
长牡蛎AMPK基因在三倍体性腺发育中的表达特性及调控机制
Expression Characteristics and Regulation Mechanism of AMPK Gene in Gonad Development of Triploid Pacific Oyster(Crassostrea gigas)
摘要
Abstract
To explore the molecular regulation mechanism of gonadal development in triploid Crassostrea gigas,the expression characteristics of AMPK gene and its target genes during gonadal development in C.gigas were investigated.AMPK contained three subunits,AMPK α,AMPK β and AMPK y.The results of real time PCR showed that the three AMPK subunits mRNA all showed a decreasing trend during the gonadal development in diploid C.gigas and an increasing trend in triploid C.gigas.The phosphorylation level of AMPK α at Thr172(p-AMPK α)of gonadal development in diploid C.gigas was high at the proliferative stage and almost completely disappeared by the mature stage,indicating that AMPK may play an important regulatory role in the early stage of gonadal development in C.gigas,while in triploid C.gigas,the level of p-AMPK a was significantly higher in sterile triploids(3nβ)than in fertile triploids(3nα),speculating that p-AMPK α enhancement might be closely related to triploid infertility.AMPK α active protein was mainly located in follicular cells,spermatogonia/spermatocytes and oogonia/oocytes,suggesting that this protein may be involved in the regulation of the early gametogenesis of C.gigas.The expression of potential AMPK targeting genes(GCS,CREB,SREBP1,ACC and Raptor)were analyzed,and the results showed that p-AMPK αmay be involved in the regulation of CREB and SREBP1 expression.It is speculated that AMPK may promote glycogen and lipid breakdown and provide energy for germ cell division and differentiation at the early stage of gonadal development in fertile triploid C.gigas.In the sterile triploid C.gigas,the expression of AMPK was not inhibited due to the lack of vigorous germ cell division,which maintained the energy homeostasis in the body.关键词
长牡蛎/性腺发育/AMPK基因/三倍体/调控机制Key words
Crassostrea gigas/gonadal development/AMPK gene/triploid/regulation mechanism分类
水产学引用本文复制引用
王朔,于红,李琪,王庆志..长牡蛎AMPK基因在三倍体性腺发育中的表达特性及调控机制[J].中国海洋大学学报（自然科学版）,2024,54(1):48-57,10.基金项目
国家自然科学基金项目(31672649) （31672649）
国家重点研究发展计划项目(2018YFD0900200) （2018YFD0900200）
辽宁省海洋经济发展专项(202222) （202222）
大连市科技创新基金项目(2021YF16SN015,2021JB11SN035)资助 Supported by the National Natural Science Foundation of China(31672649) （2021YF16SN015,2021JB11SN035）
the National Key Research and Development Program of China(2018YFD0900200) （2018YFD0900200）
the Ocean Economic Development Grants of Liaoning Province(202222) （202222）
the Science and Technology Foundation of Dalian(2021YF16SN015,2021JB11SN035) （2021YF16SN015,2021JB11SN035）
