长牡蛎AMPK基因在三倍体性腺发育中的表达特性及调控机制OACSTPCD

To explore the molecular regulation mechanism of gonadal development in triploid Crassostrea gigas,the expression characteristics of AMPK gene and its target genes during gonadal development in C.gigas were investigated.AMPK contained three subunits,AMPK α,AMPK β and AMPK y.The results of real time PCR showed that the three AMPK subunits mRNA all showed a decreasing trend during the gonadal development in diploid C.gigas and an increasing trend in triploid C.gigas.The phosphorylation level of AMPK α at Thr172(p-AMPK α)of gonadal development in diploid C.gigas was high at the proliferative stage and almost completely disappeared by the mature stage,indicating that AMPK may play an important regulatory role in the early stage of gonadal development in C.gigas,while in triploid C.gigas,the level of p-AMPK a was significantly higher in sterile triploids(3nβ)than in fertile triploids(3nα),speculating that p-AMPK α enhancement might be closely related to triploid infertility.AMPK α active protein was mainly located in follicular cells,spermatogonia/spermatocytes and oogonia/oocytes,suggesting that this protein may be involved in the regulation of the early gametogenesis of C.gigas.The expression of potential AMPK targeting genes(GCS,CREB,SREBP1,ACC and Raptor)were analyzed,and the results showed that p-AMPK αmay be involved in the regulation of CREB and SREBP1 expression.It is speculated that AMPK may promote glycogen and lipid breakdown and provide energy for germ cell division and differentiation at the early stage of gonadal development in fertile triploid C.gigas.In the sterile triploid C.gigas,the expression of AMPK was not inhibited due to the lack of vigorous germ cell division,which maintained the energy homeostasis in the body.