鹅颈藤壶多糖提取、理化性质及N-糖链表达谱分析研究OACSTPCD

To extract polysaccharide from Lepas anatifera,a gooseneck barnacle,in the South China Sea.Based on the analysis of its basic physical and chemical properties,the expression profile of N-gly-cans in Gooseneck barnacle at different growth stages was compared.Polysaccharide was extracted by degreasing,alkaline hydrolysis and ethanol precipitation.The monosaccharide composition,molecular distribution,total polysaccharide content and protein content were analyzed by high performance liquid chromatography,high performance gel permeation chromatography,pheno-lsulfuric acid method and Coomassie brilliant blue method.Liquid mass analysis(PGC-ESI-MS/MS)was used to analyze and compare the N-glycans in the musculature stalk of adult(A-GB)and larvae(L-GB)barnacles.The mo-lecular weight of polysaccharide in muscle stalk was 18.40 kDa.The total polysaccharide content is 11.99％,the protein content is 60.14％,rich in mannose,glucose,galactose amino,among which the content of mannose is up to 80％.Twenty-six N-glycans were identified in both adult and larvae of bar-nacles.The high mannose-type N-glycans accounted for 20.81％of the total glycans in adults,and the glycotypes with high relative abundance were F1H3N2(39.06％)、F1 H3N3(24.44％)、H3N3(9.35％).The high mannose-type N-glycans accounted for 52.17％of the total N-glycans in the larvae,and the glycotypes with high relative abundance were F1H3N2(21.65％)、H6N2(15.93％)、H5N2(14.75％).Barnacle polysaccharide is a kind of N-glycosylated protein complex.It is speculated that N-glucans plays a crucial role in the growth and development of barnacle,and high mannose-type N-glycosyl chain may be closely related to its adhesion ability.