中国海洋大学学报（自然科学版）2024，Vol.54Issue(1)：95-103,9.DOI:10.16441/j.cnki.hdxb.20220457
鹅颈藤壶多糖提取、理化性质及N-糖链表达谱分析研究
Gooseneck Barnacle Polysaccharide Extraction,Physicochemical Properties and N-Glycans Expression Spectrum Analysis
摘要
Abstract
To extract polysaccharide from Lepas anatifera,a gooseneck barnacle,in the South China Sea.Based on the analysis of its basic physical and chemical properties,the expression profile of N-gly-cans in Gooseneck barnacle at different growth stages was compared.Polysaccharide was extracted by degreasing,alkaline hydrolysis and ethanol precipitation.The monosaccharide composition,molecular distribution,total polysaccharide content and protein content were analyzed by high performance liquid chromatography,high performance gel permeation chromatography,pheno-lsulfuric acid method and Coomassie brilliant blue method.Liquid mass analysis(PGC-ESI-MS/MS)was used to analyze and compare the N-glycans in the musculature stalk of adult(A-GB)and larvae(L-GB)barnacles.The mo-lecular weight of polysaccharide in muscle stalk was 18.40 kDa.The total polysaccharide content is 11.99％,the protein content is 60.14％,rich in mannose,glucose,galactose amino,among which the content of mannose is up to 80％.Twenty-six N-glycans were identified in both adult and larvae of bar-nacles.The high mannose-type N-glycans accounted for 20.81％of the total glycans in adults,and the glycotypes with high relative abundance were F1H3N2(39.06％)、F1 H3N3(24.44％)、H3N3(9.35％).The high mannose-type N-glycans accounted for 52.17％of the total N-glycans in the larvae,and the glycotypes with high relative abundance were F1H3N2(21.65％)、H6N2(15.93％)、H5N2(14.75％).Barnacle polysaccharide is a kind of N-glycosylated protein complex.It is speculated that N-glucans plays a crucial role in the growth and development of barnacle,and high mannose-type N-glycosyl chain may be closely related to its adhesion ability.关键词
鹅颈藤壶多糖/N糖链/高甘露糖型/表达谱究Key words
gooseneck barnacle polysaccharide/N-glycans/high mannose type/comparative study of difference investigate分类
中医学引用本文复制引用
王茹,姜媛媛,林川,潘琳,管守德,于广利,吕友晶,李国云..鹅颈藤壶多糖提取、理化性质及N-糖链表达谱分析研究[J].中国海洋大学学报（自然科学版）,2024,54(1):95-103,9.基金项目
国家自然科学基金项目(31600646) （31600646）
泰山学者攀登计划项目(TSPD20210304) （TSPD20210304）
山东省重大科技创新工程项目(2021ZDSYS22)资助 Supported by the National Natural Science Foundation of China(31600646) （2021ZDSYS22）
the Mount Tai Scholar Climbing Program(TSPD20210304) （TSPD20210304）
the Major Scientific and Technological Innovation Project of Shandong Province(2021 ZDSYS22) （2021 ZDSYS22）
