热带作物学报2023，Vol.44Issue(12)：2384-2391,8.DOI:10.3969/j.issn.1000-2561.2023.12.003
木薯查尔酮合酶MeCHS基因家族特征与表达分析
Characteristics and Expression Analysis Of Chalcone Synthase MeCHS Family in Cassava
摘要
Abstract
Chalcone synthase(CHS)is the first limiting enzyme in flavonoid synthesis pathway and plays an important role in flower color formation,growth and development,and abiotic stress.In order to clarify the characteristics of cas-sava MeCHS gene family and its expression in cassava postharvest physiological deterioration(PPD),the MeCHS gene family was analyzed using bioinformatics,including physicochemical property and subcellular location.qRT-PCR was carried out to detect the expression levels of MeCHS genes in different varieties,different tissues and PPD process.The genes with significant relative expression were selected for cloning and subcellular localization verification.A total of 5 MeCHS gene members were identified in the cassava genome.The secondary structure analysis showed that the MeCHS gene family was mainly composed of α-helices and random coils.Tissue specific expression indicated that the expres-sion levels of MeCHS genes were higher in stems.The major expressed MeCHS genes in cassava were MeCHS1,MeCHS2 and MeCHS3;and the expression levels of these three genes were gradually increase with PPD.MeCHS1,MeCHS2 and MeCHS3 were successfully cloned;and fluorescence transient expression showed that MeCHS1,MeCHS2 and MeCHS3 were localized in the cytoplasm,which was consistent with the predicted results.This study will provide a theoretical basis for further revealing the function of MeCHS gene family and improving the PPD tolerance of cassava.关键词
木薯/查尔酮合酶/qRT-PCR/克隆/表达分析Key words
cassava/chalcone synthase/qRT-PCR/cloning/expression analysis分类
农业科学引用本文复制引用
安飞飞,齐剑雄,陈松笔,罗秀芹,蔡杰,薛晶晶..木薯查尔酮合酶MeCHS基因家族特征与表达分析[J].热带作物学报,2023,44(12):2384-2391,8.基金项目
中央级公益性科研院所基本科研业务费专项(No.1630052023016) （No.1630052023016）
海南省自然科学基金项目(No.320MS100). （No.320MS100）
