木薯查尔酮合酶MeCHS基因家族特征与表达分析OACSCDCSTPCD

Chalcone synthase(CHS)is the first limiting enzyme in flavonoid synthesis pathway and plays an important role in flower color formation,growth and development,and abiotic stress.In order to clarify the characteristics of cas-sava MeCHS gene family and its expression in cassava postharvest physiological deterioration(PPD),the MeCHS gene family was analyzed using bioinformatics,including physicochemical property and subcellular location.qRT-PCR was carried out to detect the expression levels of MeCHS genes in different varieties,different tissues and PPD process.The genes with significant relative expression were selected for cloning and subcellular localization verification.A total of 5 MeCHS gene members were identified in the cassava genome.The secondary structure analysis showed that the MeCHS gene family was mainly composed of α-helices and random coils.Tissue specific expression indicated that the expres-sion levels of MeCHS genes were higher in stems.The major expressed MeCHS genes in cassava were MeCHS1,MeCHS2 and MeCHS3;and the expression levels of these three genes were gradually increase with PPD.MeCHS1,MeCHS2 and MeCHS3 were successfully cloned;and fluorescence transient expression showed that MeCHS1,MeCHS2 and MeCHS3 were localized in the cytoplasm,which was consistent with the predicted results.This study will provide a theoretical basis for further revealing the function of MeCHS gene family and improving the PPD tolerance of cassava.