木薯MNP标记在品种鉴定中的应用OACSCDCSTPCD

MNP(multiple nucleotide polymorphism,MNP)is a new molecular marker technology emerged with the development of molecular marker technology.While genotyping by target sequencing(GBTS)amplifies only one SNP site in one amplicon,MNP can amplify multiple SNP sites simultaneously in one amplicon based on GBTS.This tech-nology has the features of low cost,high detection efficiency,flexible application and wide adaptability,Compared with SNP,MNP has better ability to differentiate varieties.In order to establish a rapid,accurate and efficient method for cassava variety identification and to screen MNP molecular markers suitable for cassava variety identification,this study screened highly polymorphic regions and designed primers in the whole genome range,and finally obtained 623 cassava MNP marker loci in the whole genome range,the cassava MNP markers were evaluated using 28 cassava varie-ties.The results showed that the MNP marker typing reproducibility was up to 100%.4.07±1.68 alleles were detected in 28 cassava varieties,with a maximum of 12 alleles.When all cassava varieties were compared one by a one,99.47%(376/378)of the differences between pairs were greater than 46%,with the proportion ranging from 0.3%to 81.0%,with a mean value of 71.78%.Meanwhile,MNP markers can be used for identification in the breeding process.In conclusion,the cassava MNP molecular markers developed in this study have high reproducibility,polymorphism and variety dif-ferentiation ability,and could be widely used for research on germplasm diversity,new variety breeding and variety identification of cassava.