热带作物学报2023，Vol.44Issue(12)：2469-2476,8.DOI:10.3969/j.issn.1000-2561.2023.12.012
玉竹蔗糖合成酶(SUS)基因家族的鉴定、表达分析及PoSUS1基因的克隆
Identification and Expression Profiling of the Sucrosesynthase(SUS)Gene Family and Cloning of PoSUS1 in Polygonatum odoratum
摘要
Abstract
Polysaccharide is a quality marker of Polygonatum odoratum,which has significant pharmacological effects in immune regulation and anti-tumor.As one of the key enzymes in polysaccharide synthesis,sucrosesynthase(SUS)has always been an important research field to reveal the synthesis of plant polysaccharides.Based on transcriptom data of Polygonatum odoratum,the SUS gene family members were identified by bioinformatics,and their expression pro-filing were analyzed using qPCR.The results showed that eight PoSUS gene were identified,their protein ranged from 111 to 310 amino acid residues(aa)in length,and relative molecular weight varied from 12.81 kDa to 35.43 kDa,isoelectric point(pI)in the range of 5.83 to 9.18;phylogenetic analysis indicated that eight PoSUS genes were divided into 3 subfamily,and the subfamily Ⅲ included the largest PoSUS genes family member;subcellural localization analysis showed that most of PoSUS proteins were located in chloroplast.Additionally,expression patterns analysis revealed that PoSUS1 and PoSUS6 genes were preferably expressed in rhizome,and the transcript levels of PoSUS1 and PoSUS2 were higher in high-polysaccharide cultivar HN1 than those in a low-polysaccharide cultivar AH2.In addition,the CDS of PoSUS1 gene were cloned from HN1 and AH2,there are three amino acid difference between HN1 and AH2,which located in sucrose synthase domain.Our findings can laid a basis for the further study of functional analysis of SUS genes,and provided a theoretical basis for analyzing molecular mechanism of the formation mechanism of me-dicinal quality of Polygonatum odoratum.关键词
玉竹/SUS基因家族/表达分析/PoSUS1Key words
Polygonatum odoratum/SUS gene family/expression analysis/PoSUS1分类
农业科技引用本文复制引用
潘根,秦裕辉,张水寒..玉竹蔗糖合成酶(SUS)基因家族的鉴定、表达分析及PoSUS1基因的克隆[J].热带作物学报,2023,44(12):2469-2476,8.基金项目
湖南省科技成果转化及产业化计划项目(No.2020SK2029) （No.2020SK2029）
湖南省中医药科研计划项目(No.A2022002,No.A2022005). （No.A2022002,No.A2022005）
