热带作物学报2023，Vol.44Issue(12)：2514-2519,6.DOI:10.3969/j.issn.1000-2561.2023.12.017
水心病菠萝果肉组织石蜡切片显微观察与分析
Analysis and Microscopic Observation of Paraffin Sections of Pineap-ple Pulp in Watercore
摘要
Abstract
Watercore is a kind of physiological disease of pineapple fruit,which causes the intercellular space of pine-apple pulp tissue to be filled with cell fluid and appear as water-soaked,which seriously affects the edible value and commercial value of the fruit.Watercore has troubled the development of pineapple industry for many years,but its mechanism is not clear.In order to explore the pathogenesis of watercore in pineapple,this study took the main pineap-ple cultivar Comte de paris as the material,the paraffin section method was used to observe the process of watercore in pineapple fruit and the different tissue parts of normal and watercore fruit(flower stock,carpel,fruit sequence axis).The results showed that the cell structure of pineapple pulp was closely related to watercore:the cell structure of normal fruit pulp was complete,the cell space was obvious,the vascular bundle was intact,the phloem cells were arranged tightly and neatly,and the xylem ducts were arranged regularly.However,fruit flesh cells in watercore are squeezed and deformed,and the cell wall rupture results in incomplete cell structure,destruction of vascular bundle morphology,ex-trusion and deformation of phloem,and destruction of xylem ducts to form a cavity.With the deepening of the onset process of watercore,the xylem cavity becomes larger.Through the morphological observation of different tissue parts of normal fruits and fruit with watercore,it was found that the changes of cell structure caused by watercore in different tissue parts were basically the same.There were obvious differences in vascular bundle morphology and structure in different tissue parts.The vascular bundles in the fruit sequence axis and receptacle were in accordance with the typical characteristics of vascular bundles in monocotyledons.The vascular bundles were surrounded by a sheath composed of thick-walled mechanical tissue at the periphery of each vascular bundle.On the other hand,there were more sachmatous cells at both ends of the vascular bundle.The inner part of the vascular bundle sheath contains primary phloem and primary xylem without the middle bundle cambium.The vascular bundle structure of the carpel was atypical,and most of the vascular bundles were not surrounded by vascular sheath.Vascular tissues in the fruit sequence axis were the most developed,followed by receptacle,and vascular tissues in the carpel were the least developed.These results revealed the morphological changes of pineapple watercore during its occurrence and provided reference for further research on the mechanism of watercore.关键词
菠萝/水心病/细胞结构/石蜡切片/维管束Key words
pineapple/watercore/cell structure/paraffin section/vascular bundle分类
农业科技引用本文复制引用
姚艳丽,吴严,李明伟,付琼,刘胜辉,朱祝英,张秀梅..水心病菠萝果肉组织石蜡切片显微观察与分析[J].热带作物学报,2023,44(12):2514-2519,6.基金项目
海南省基础与应用基础研究计划(自然科学领域)高层次人才项目(No.2019RC287) （自然科学领域）
广东省自然科学基金项目(No.2019A1515011186) （No.2019A1515011186）
广东省现代农业产业技术体系创新团队建设专项资金(No.2023KJ109). （No.2023KJ109）
