热带作物学报2023，Vol.44Issue(12)：2569-2578,10.DOI:10.3969/j.issn.1000-2561.2023.12.023
西沙海绵共附生放线菌ITBB-ZK-a5的分离鉴定及抗菌活性研究
Isolation,Identification and Anti-phytopathogenic Fungi Activity of Symbiotic Actinomycetes from Xisha Sponge
摘要
Abstract
Marine actinomycetes possess great capacity in producing special metabolites due to their special environ-ment and unique properties in physiological characters and genetic background.In this study,sponge was used as isola-tion material to explore the resources of symbiotic actinomycetes with antibacterial activity.Firstly,an active actino-mycetes strain,ITBB-ZK-a5,was isolated and purified from the sample of Xisha sponge by plate separation method.The strain was identified as Streptomyces by analysis of the phylogenetic tree based on 16S rRNA gene sequence and colony morphological characteristics.Secondly,the inhibitory spectrum of strain ITBB-ZK-a5 against plant pathogenic fungi was studied by plate confrontation method.The results showed that ITBB-ZK-a5 had a broad antibacterial spec-trum,in which the 16 tested pathogenic fungi were inhibited significantly.ITBB-ZK-a5 also showed good antibacterial activity in 14 days and 30 days.In order to explore the reason why strain ITBB-ZK-a5 can inhibit the growth of patho-genic bacteria,the antibacterial activity of asepsis agar from the ITBB-ZK-a5 edge were determined,and the results showed that the antibacterial rate were negatively correlated with colony distance.This result revealed that actinomy-cetes maybe can secrete active substances to inhibit the growth of pathogenic bacteria.Thirdly,the strain had a good antibacterial stability by detecting the antimicrobial activity of five continuously dynasties.Again,light stability and thermal stability of the ITBB-ZK-a5 crude extracts from the rice solid fermentation were tested through the filter method;the results show that with the increase of bath temperature and UV duration,the antibacterial activity of the strain ITBB-ZK-a5 crude extracts were decreased.Finally,the EC50 value of strain ITBB-ZK-a5 against anthrax diseases of banana and papaya were determined.Plant diseases infected by fungi are the main agricultural diseases,and chemical pesticides are the most commonly used method in diseases control.However,the widespread use of chemical pesticides will not only enhance crop resistance to drugs,but also cause pollution to the ecological environment.More importantly,pesticide residues also have a certain influence on human health.Therefore,the common goal of the world is to find more green,efficient and safe control methods.Actinomycetes are found to have biological control effects.The symbi-otic actinomycetes isolated from sponges have an inhibitory effect on pathogenic fungi of tropical crops,which can be applied to efficient diseases control,conforming to the concept of green environmental protection.In this paper,we isolated and identified the strain ITBB-ZK-a5,symbiotic actinomycetes from South China Sea sponge,which has the activity of inhibiting pathogenic fungi of various tropical crops,and studied its antibacterial activity,providing a re-search basis for the development and utilization of biocontrol strains in the future.关键词
海绵/海洋放线菌/抗植物病原真菌/抑菌活性/生防菌株Key words
sponge/marine actinomycetes/anti-phytopathogenic fungi/antibacterial activity/bio-control strain分类
农业科技引用本文复制引用
齐敏,郭志凯,熊子君,张世清,吴炜城,李吉涛..西沙海绵共附生放线菌ITBB-ZK-a5的分离鉴定及抗菌活性研究[J].热带作物学报,2023,44(12):2569-2578,10.基金项目
海南省自然科学基金项目(No.322QN387) （No.322QN387）
生物资源保护与利用湖北省重点实验室开放基金项目(No.PKLHB1920) （No.PKLHB1920）
海南省基础与应用基础研究计划(自然科学领域)高层次人才项目(No.2019RC306). （自然科学领域）
