日用化学工业（中英文）2023，Vol.53Issue(12)：1385-1391,7.DOI:10.3969/j.issn.2097-2806.2023.12.004
复方丁香挥发油对UV致小鼠皮肤光老化损伤的保护作用
Protective effect of compound clove volatile oil on UV induced skin photoaging damage in mice
摘要
Abstract
Photoaging is a process of premature skin aging caused by prolonged exposure to ultraviolet radiation.It is characterized by sunspots,coarse wrinkles,loss of elasticity,and photochemical keratosis.The protective effect of volatile oil of compound clove on the skin of mice induced by UV was studied in this paper.Firstly,the main components of volatile oil of compound clove were analyzed by gas chromatography-mass spectrometry(GC-MS).After the back irradiation,the skin tissues of the irradiated area were stained by HE and Masson to observe the changes of the skin morphology and collagen fibers in the dermis.The expressions of collagen Ⅰ and MMP-3 in skin were detected by Western Blot.The results show that the volatile oil of compound clove contains 50 components,of which 11 components account for 83.56% of the chromatographic peak area.The main components are eugenol(31.96% ),(E)-3-butylidene-4,5-dihydroisobenzofuran-1(3H)-one(20.56% )and caffeine(12.72% ).Compared with the model group,compound clove volatile oil significantly improves the appearance and tissue structure of the photoaged mice skin,and increases collagen fibers.The results of Western Blot show that compound clove volatile oil can up-regulate the expression of typeⅠcollagen and down-regulate the expression of MMP-3 protein.In conclusion,compound clove volatile oil can improve skin wrinkles and roughness caused by ultraviolet light,inhibit collagen degradation in photoaged skin,and promote skin repair.关键词
复方丁香挥发油/气相色谱-质谱联用/化学成分/光老化Key words
compound clove volatile oil/GC-MS/chemical components/photoaging分类
化学化工引用本文复制引用
丁羽,吴毅,任明诗,李子涵,刘波..复方丁香挥发油对UV致小鼠皮肤光老化损伤的保护作用[J].日用化学工业（中英文）,2023,53(12):1385-1391,7.基金项目
国家自然科学基金资助项目(81560723) （81560723）
