日用化学工业（中英文）2023，Vol.53Issue(12)：1466-1471,6.DOI:10.3969/j.issn.2097-2806.2023.12.015
高效液相色谱-蒸发光散射法测定脂质体中神经酰胺NP和AP含量
Determination of ceramide NP and AP content in liposomes by high performance liquid chromatography-evaporative light scattering
摘要
Abstract
Simultaneous chromatographic separations of ceramide NP and ceramide AP in ceramide liposomes were carried out by high-performance liquid chromatography(HPLC)coupled to evaporative light scattering detector(ELSD).The ceramide liposomes samples were treated with methanol-isopropanol(volume ratio 7∶3)and analyzed by Agilent ZORBAX StableBond C8(4.6×150 mm,3.5 μm)column.The simultaneous separation of ceramide NP and AP was achieved by gradient elution with 0.1% formic acid in methanol-isopropanol(volume ratio 7∶3)and acetonitrile as the mobile phases.The flow rate was 1.0 mL/min and the column temperature was 40℃.Evaporator and nebulizer temperature were set at 90℃and 70℃,and the gas flow rate was 1.0 L/min.The result shows the detection limits of ceramide NP and AP in ceramide liposomes are 3 and 6 μg/mL,respectively,and the quantitative limits are 1 and 2 μg/mL,respectively.The RSD of test on ceramide NP and AP repeatability and precision are all less than 2% ,and the linear relationship of calibration curves for ceramide NP and AP is good(R2>0.999)in the mass concentration range of 50-500 μg/mL and 25-250 μg/mL,respectively.Moreover,the recovery rates range from 96.2% to 105.1% at different concentration levels.A simple,reproducible and reliable method is provided for the determination of ceramide NP and AP contents in different ceramide liposomes.关键词
神经酰胺NP/神经酰胺AP/脂质体/高效液相色谱法/蒸发光散射检测器Key words
ceramide NP/ceramide AP/liposomes/high-performance liquid chromatography/evaporative light scattering detector分类
化学化工引用本文复制引用
赵格格,苏温柔,王飞飞..高效液相色谱-蒸发光散射法测定脂质体中神经酰胺NP和AP含量[J].日用化学工业（中英文）,2023,53(12):1466-1471,6.基金项目
云南特色植物提取实验室自主研究项目基金资助(2022YKZY003) （2022YKZY003）
