山东医药2023，Vol.63Issue(35)：1-4,14,5.DOI:10.3969/j.issn.1002-266X.2023.35.001
美洲大蠊小肽预处理对H2O2诱导人卵巢颗粒细胞氧化应激及凋亡的抑制作用
Inhibition of SPPA pretreatment on H2O2-induced oxidative stress and apoptosis in human ovarian granulosa cells
摘要
Abstract
Objective To investigate the effects of pretreatment with different concentrations of small peptides from Periplaneta americana(SPPA)on H2O2-induced oxidative stress and apoptosis in human ovarian granulosa cells(KGN cells).Methods KGN cells in the logarithmic growth phase were divided into the blank group,H2O2 group and 50,100,and 150 SPPA groups.Cells in the five groups were cultured for 24 h.Cells in the blank group were not treated,and 250 μmol/L H2O2 was added to the H2O2 group for 6 h.Cells in the 50,100,and 150 SPPA groups were pretreated with 50,100,and 150 μg/mL SPPA,respectively,and then were treated with 250 μmol/L H2O2 for 6 h.CCK-8 was used to detect the cell survival rates in the blank group,H2O2 group and 50,100,and 150 SPPA groups pretreated for 6,12 and 24 h,respectively,and we compared the expression levels of reactive oxygen species(ROS),nitric oxide(NO)and malo-ndialdehyde(MDA)in the cells of all groups(50,100,and 150 SPPA groups were all pretreated for 12 h).Results The cell survival rate of the H2O2 group was lower than that of the blank group(P＜0.05).The differences in cell survival rates between the 50,100 and 150 SPPA groups pretreated for 6 h and the H2O2 group were not statistically significant(all P＞0.05),and the cell survival rates of the 50,100 and 150 SPPA groups pretreated for 12 and 24 h were higher than those of the H2O2 group(all P＜0.05).The cell survival rates of the 50,100 and 150 SPPA groups at 12 and 24 h of pre-treatment were higher than those at 6 h of pretreatment(all P＜0.05);no significant differences were found in the survival rates between the 50,100 and 150 SPPA groups at 12 and 24 h of pretreatment(all P＞0.05).Compared with the blank group,the expression levels of MDA,NO and ROS in cells of the H2O2 group increased(all P＜0.05);compared with the H2O2 group,the expression levels of MDA,NO and ROS in cells of the 50,100 and 150 SPPA groups decreased(all P＜0.05),and no significant differences were found between the 50,100 and 150 SPPA groups(all P＞0.05).Conclusion SPPA pretreatment was able to reduce H2O2-induced oxidative stress and apoptosis in KGN cells,and 50 μg/mL for 12 h was the optimal condition.关键词
美洲大蠊小肽/过氧化氢/氧化应激/细胞凋亡/人卵巢颗粒细胞Key words
small peptides from Periplaneta americana/H2O2/oxidative stress/apoptosis/human ovarian granu-losa cells分类
医药卫生引用本文复制引用
孙蕊仙,唐运萍,杨礼莲,伏蓉,王婧羽,隋世燕..美洲大蠊小肽预处理对H2O2诱导人卵巢颗粒细胞氧化应激及凋亡的抑制作用[J].山东医药,2023,63(35):1-4,14,5.基金项目
国家自然科学基金资助项目(32260865,31760719) （32260865,31760719）
云南省科技厅基础研究计划(202101BA070001-202) （202101BA070001-202）
云南省科技人才与平台计划(202305AF150083). （202305AF150083）
